A series of prior reports [l, 2, 31 have established the structure of esters produced by gram-negative micrococci capable of profuse growth on n-alkanes. The purpose of this paper is to describe the general properties and physiology of the organisms. All strains of micrococci included in this study have been allocated to a new species : Micrococcus cerificans sp. n. Materials and MethodsThe organisms were isolated from various soils by inoculating 100 ml. of mineral medium amended with 1 yo of the appropriate alkane or olefin. The mineral medium employed was essentially that described by DWORKIN and FOSTER [a] except that the phosphate content was increased five-fold. The isolation technique was as follows: basal medium amended with hydrocarbon was inoculated with about 10 grams of soil and incubated for 6 days on the gyrotory shaker a t room temperature. Transfer (2 ml.) was made to fresh minerah-hydrocarbon medium and the subculture was incubated for 48 hours at which time a transfer again was made to new medium. At least 6, commonly more, such transfers were made before the cultures were streaked for isolation on solid media. Actual isolation of the organisms was carried out by SOHNQEN'S technique [6] using the designated hydrocarbon as substrate. Under these conditions of enrichment (23 transfers in one experiment) gram-negative micrococci were the predominant population but colonies of Achrmobacter, Nocardia, coryneform organisms, and Pseudomonas were also present. Some difficulty in purification of cultures was encountered, probably due to association of severi
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