Alkane‐Oxidizing Micrococci

Finnerty, W. R.; Hawtrey, Elizabeth; Kallio, R. E.

doi:10.1002/jobm.3630020302

Cited by 36 publications

(15 citation statements)

References 9 publications

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“…The organism used in this study, Micrococcus cerijicans, was isolated by enrichment culture with the use of hexadecane (Finnerty, Hawtrey & Kallio, 1962) from soil. It might be possible to isolate an organism from soil which would more readily utilize, or even prefer, plant wax paraffin, if such paraffins were used in the enrichment procedures.…”

Section: -3mentioning

confidence: 99%

Metabolism of a Plant Wax Paraffin (n-Nonacosane) by a Soil Bacterium (Micrococcus cerificans)

Hankin

Kolattukudy

1968

Journal of General Microbiology

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S U M M A R YLong-chain paraffins, such as nonacosane, reach the soil from plants and are degraded by micro-organisms. The ability of one soil organism, Micrococcus ceriJicans, to grow on cabbage paraffin (n-nonacosane) was studied. Unlike the pattern seen with hexadecane, waxy esters were not produced when nonacosane was the sole carbon source, but esters were formed when a cooxidation technique of "*C] n-nonacosane with hexadecane was employed. The alcohol and acid portions of the radioactive esters formed were primarily of chain length Cis, G7 and C18; neither nonacosanol nor nonacosanoic acid were detected. Thus the nonacosanol and nonacosanoic acid which might have been produced from n-nonacosane must have been oxidized to shorterchain compounds which in turn were incorporated into waxy esters as well as other lipids.

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Section: -3mentioning

confidence: 99%

Metabolism of a Plant Wax Paraffin (n-Nonacosane) by a Soil Bacterium (Micrococcus cerificans)

Hankin

Kolattukudy

1968

Journal of General Microbiology

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“…strain HO1-N and A. calcoaceticus BD413 were previously described (8,12,21). Rifampicinresistant mutants of each strain were isolated after nitrosoguanidine mutagenesis (1).…”

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confidence: 99%

Transformation and mobilization of cloning vectors in Acinetobacter spp

Singer¹,

Tuijl²,

Finnerty³

1986

J Bacteriol

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, and RK2-derived plasmids were introduced into Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by transformation and conjugal mobilization. The transformation frequencies of BD413 were 4.2 x 106 to 6.3 x 106 transformants per ,ug of DNA per 109 recipient cells. Conjugal mobilization frequencies were 1.1 x 10-1 to 8.5 x 10-1 per recipient. An improved method for the transformation of A. calcoaceticus BD413 is reported.R300B-, R1162-, and RSF1010-based vectors have been used for gene cloning in Pseudomonas (2)(3)(4)(5)(18)(19)(20) and Methylophilus (23) spp. Plasmid pRK290 is a 20-kilobase (kb) broad-host-range RK2 derivative that can be mobilized into a number of gram-negative organisms, including Acinetobacter spp. (9). A previous report suggested that transformation might be used to introduce plasmid cloning vehicles into Acinetobacter strains (10). We examined plasmid transformation in Acinetobacter sp. strain HO1-N and Acinetobacter calcoaceticus BD413 by using a variety of broadhost-range vectors and transformation protocols, and we have optimized conditions for plasmid transformation. We examined conjugal mobilization as an alternative to transformation for the introduction of cloning vectors into Acinetobacter spp.Acinetobacter sp. strain HO1-N and A. calcoaceticus BD413 were previously described (8,12,21). Rifampicinresistant mutants of each strain were isolated after nitrosoguanidine mutagenesis (1). Escherichia coli HB101, HB1O1(pRK290), and HB101(pRK2013) were obtained from Donald R. Helinski (9, 11). Plasmids R300B and pTB107, an R300B derivative carrying a 1.5-kb kanamycin resistance determinant, were obtained from Peter T. Barth (5,6,14,23). Plasmids RSF1010, pKT230, pKT231, and pKT248 were obtained from K. N. Timmis (2-4).L broth (pH 7.2) consisted of 1% tryptone, 0.5% yeast extract, and 0.5% NaCl. Solid medium contained 1.6% agar. Antibiotics were added to L agar at the following final concentrations: rifampin, 100 ,ug/ml; kanamycin, 50 ,ug/ml; tetracycline, 15 ,ug/ml; streptomycin, 15 ,ug/ml; sulfadiazine, 250 ,ug/ml; and chloramphenicol, 50 ,ug/ml. Recipient Acinetobacter cell cultures were grown overnight in shaken, 50-ml L-broth cultures at 30°C. Cultures were diluted to approximately 107 cells per ml with L broth and grown for 2 to 6 h at 30°C, and samples of the cultures were used according to the specified transformation protocols.Plasmid DNA was isolated by an alkaline sodium dodecyl sulfate extraction procedure (17) or by the procedure of Clewell and Helinski (7), with 0.5% Triton X-100 substituted for Brij 58 and deoxycholate. Plasmids were purified by CsCl-ethidium bromide density gradient ultracentrifugation.The transformation procedures examined were those of * Corresponding author. Mandel and Higa (16), Lederberg and Cohen (15), Kushner (13), and Eichenlaub and Steinbach (10). Transformations were carried out for 1 h and were terminated by the addition of DNase to 5 ,ug/ml. Transformants and exconjugants carrying R300B, pTB107, and pRK290 were selected on L-agar...

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“…Table 7), additionally utilise middlechain n-alkanes (C 6 -C 9 ) as a sole source of carbon, provided that the hydrocarbons are supplied in the vapour phase (Kleber et aI., 1983;Eremina et aI., 1987). Finnerty et al, 1962;(4) Fewson, 1967; …”

Section: Substrate Specificity Product Patterns and Degradation Pathwaymentioning

confidence: 99%

The Biology of Acinetobacter

Towner¹,

Bergogne-Bérézin²,

Fewson³

1991

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All rights reservedNo part of this book may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording, or otherwise, without written permission from the Publisher PREFACEThe 1st International Workshop on Acinetobacter was held on 6th September, 1986, in Manchester, England, in association with the 14th International Congress of Microbiology. That occasion was so well attended and productive that there were soon discussions about how, when and where the next meeting should be held. This time, however, there was sufficient confidence to think of a more substantial meeting and to plan for the proceedings to be published. It emerged that there was wide agreement that the time was ripe to take stock of the entire biology of Acinetobacter: its occurrence and taxonomy; its molecular biology, biochemistry and physiology; its clinical importance and its industrial and commercial applications. The 2nd International Workshop on Acinetobacter took place from 6th to 7th September, 1990, at the Institut Pasteur, Paris, and was sponsored by the Federation of European Microbiological Societies. There were about 100 participants from 19 countries. The backbone of the meeting consisted of 23 plenary lectures. There were 28 posters and the meeting closed with a general discussion which went on long after the official finishing time despite all the counter-attractions of a sunny Parisian Friday afternoon. Indeed discussions continued while cruising along the Seine and while dining at the top of the Tour Montparnasse. However, the vitality and usefulness of even the most successful meeting is difficult to transmit by the printed word. The editors decided, therefore, not simply to publish the proceedings of the Workshop, but rather to commission a set of review articles with the aim of giving an up-to-date and rounded picture of our current knowledge of the genus Acinetobacter. These reviews, together with a

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Alkane‐Oxidizing Micrococci

Cited by 36 publications

References 9 publications

Metabolism of a Plant Wax Paraffin (n-Nonacosane) by a Soil Bacterium (Micrococcus cerificans)

Metabolism of a Plant Wax Paraffin (n-Nonacosane) by a Soil Bacterium (Micrococcus cerificans)

Transformation and mobilization of cloning vectors in Acinetobacter spp

The Biology of Acinetobacter

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