In vitro developtrtent oj' imrnature embryos oJ Lupinus albus L. and Lupinus mutabilis Sweet by culture of pods, ovules or isolated embryos. In Lupinus rnutabilis Sweet and Lupinus albus L., embryos of I mm or more germinated in vitro on solid media without hormones to grow. An attempt was made for smaller embryos of L. albus by culturing pods, ovules or half ovules containing endosperm and embryo, in liquid or on solid media. Pod culture in liquid medium and ovule culture on solid medium did not allow embryo development. However, embryos could be grown either by culturing ovules on a paper bridge over liquid medium or by embedding half ovules in a medium with a lowered agar concentration (5 gll).
d'hybridation interspécifique entre Lupinus albus L. et Lupinus mutabilis Sweet. Influence des conditions de culture et du génotype. Agronomie, EDP Sciences, 1986, 6 (10), pp.919-924. hal-00884840
Mutagenic treatment of seeds with Texas male-sterile cytoplasm, with gamma rays or ethyl methyl sulfonate, allowed us to obtain male-fertile maize resistant to Helminthosporium maydis race T. These two traits were transmitted through the maternal parent. The study of the native mitochondrial D NA of 5 resistant male-fertile progenies showed that the 2.35 kb molecule of D NA which characterized the N, C and S cytoplasms was absent from our mutants just as it was from the T cytoplasm. Like the revertants obtained by in vitro culture, the malc-fertile resistant A types showed a native mitochondrial Drvn which migrated in the same way as Texas type. The electrophoretic patterns of the mitochondrial D NA digested by various restriction enzymes belonged to the Normal type ; however, the material obtained by in vitro culture showed electrophoretic patterns of Texas or intermediary type according to the enzymes used. Different hypotheses arc discussed to explain these phenomena.
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