Développement <i>in vitro</i> d'embryons immatures de Lupinus albus L. et de Lupinus mutabilis Sweet par culture de gousses, d'ovules ou d'embryons isolés

Vuillaume, Elisabeth; Hoff, Tine

doi:10.1051/agro:19861008

Cited by 4 publications

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“…In the genus Lupinus some experiments were performed to establish requirements for in vitro vegetative propagation and embryo rescue culture and they were focused mainly on kind of media and their chemical components, especially growth hormones [18][19][20][21]. The present studies were aimed on establishing medium and physical conditions for pea and lupin Tab.…”

Section: Discussionmentioning

confidence: 99%

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

et al. 2013

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Pea and lupin plants have been potentially rich sources of protein for human and animal diets. Currently, in Poland the main source of protein is soya cake, imported to Poland in amount of 2 mln tons per year. For substitution of soya with domestic protein plants breeding methods for higher and stable yield of grain legumes should be developed. About 12 generations are needed to develop a new cultivar and under central or north European climate conditions only one generation per year is feasible in the field. For that reason the development of new tools is needed to accelerate obtaining homozygosity in breeding of pea and lupins. Homozygosity can be attained by selfing successive generations or by hapoidization of hybrids using different in vitro techniques and doubling chromosomes in haploid plants (for review see, e.g., [1][2][3]). Pisum and Lupinus species are known to be recalcitrant to in vitro culture [4][5][6][7]. In spite of numerous studies focused on the production of doubled haploids (DH), no important results have been noted to date [8][9][10][11]. For shortening the breeding cycle, the single seed descent technique (SSD) in combination with in vitro culture of immature embryos may be an alternative to the DH system. Currently, SSD populations are frequently used as alternatives to DH populations in genetic and genomic studies (see, for example, [12,13]). The SSD technique connected with embryo in vitro culture allows the attainment of homozygous lines in a relatively short time. Such an approach applied to the development of winter barley SSD lines enabled the shortening of the generation cycle to ca. 4 months (including verbalization) [14]. Ochatt et al. [15] proposed the reduction of pea generation cycles based on in vitro culture of embryos and a greenhouse strategy, but no information was found on embryo culture for accelerating breeding process in lupins.The aim of the present studies was to establish in vitro conditions for the culture of pea, and narrow-leafed and yellow lupin embryos as the first step in research aimed at shortening generation cycles in the SSD technique used to accelerate the development of homozygous populations. Material and methodsMaterial for the studies consisted of two pea cultivars (Pisum sativum L.) -Cysterski and Akord, two narrow-leafed lupin cultivars (Lupinus angustifolius L.) -Kadryl and Sonet, and two yellow lupin cultivars (L. luteus L.) -Mister and Taper. Pea and lupin cultivar seeds were sown in the experimental field at Wiatrowo near Poznań. Pods from each pea and lupin cultivar were detached 21-28 days after flowering, when seeds were still-green but fully developed. Seeds taken out of pods were sterilized by successive dips into ethanol 70% (3 min) and AbstractThe aim of the studies was to establish in vitro conditions for the culture of pea and lupin embryos as the first step in the development of an in vitro assisted single seed descent technique for the attainment of homozygous populations. Materials for the study included of pea, and narrow-leafed a...

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Section: Discussionmentioning

confidence: 99%

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

et al. 2013

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Lupinus

Wolko

Clements²,

Naganowska³

et al. 2010

Wild Crop Relatives: Genomic and Breeding Resources

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Somatic embryogenesis of agriculturally important lupin species (Lupinus angustifolius, L. albus, L. mutabilis)

Nadolska‐Orczyk

1992

Plant Cell Tiss Organ Cult

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Somatic embryos were obtained from immature cotyledons of Lupinus angustifolius, L. albus and L. mutabilis but not from L. luteus. Different kinds of basal media and plant growth regulators in primary and secondary culture were tested. The best induction media were based on BS'and were supplemented with 5 mg 1-1 2,4-D alone or with 0.25 mg 1-1 kinetin. Mature stage somatic embryos were obtained on media containing ABA (0.1-0.5 mg 1 -~) and a high NH4/NO 3 ratio. Embryo germination and plantlet development occurred on MS media supplemented with glutamine or GA 3.

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Développement in vitro d'embryons immatures de Lupinus albus L. et de Lupinus mutabilis Sweet par culture de gousses, d'ovules ou d'embryons isolés

Cited by 4 publications

References 1 publication

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

Preliminary results of in vitro culture of pea and lupin embryos for the reduction of generation cycles in single seed descent technique

Lupinus

Somatic embryogenesis of agriculturally important lupin species (Lupinus angustifolius, L. albus, L. mutabilis)

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