Durum wheat grains (Triticum turgidum L. ssp. durum) are the main source for the production of pasta, bread and a variety of products consumed worldwide. The quality of pasta is mainly defined by the rheological properties of gluten, an elastic network in wheat endosperms formed of gliadins and glutenins. In this study, the allelic variation at five glutenin loci was analysed in 196 durum wheat genotypes. Two loci (Glu-A1 and Glu-B1), encoding for high-molecular-weight glutenin subunits (HMW-GS), and three loci (Glu-B2, Glu-A3 and Glu-B3), encoding for low molecular weight glutenin subunits (LMW-GS), were assessed by SDS-PAGE. The SDS-sedimentation test was used and the grain protein content was evaluated. A total of 32 glutenin subunits and 41 glutenin haplotypes were identified. Four novel alleles were detected. Fifteen haplotypes represented 85.7% of glutenin loci variability. Some haplotypes carrying the 7 + 15 and 7 + 22 banding patterns at Glu-B1 showed a high gluten strength similar to those that carried the 7 + 8 or 6 + 8 alleles. A decreasing trend in grain protein content was observed over the last 85 years. Allelic frequencies at the three main loci (Glu-B1, Glu-A3 and Glu-B3) changed over the 1915–2020 period. Gluten strength increased from 1970 to 2020 coinciding with the allelic changes observed. These results offer valuable information for glutenin haplotype-based selection for use in breeding programs.
Four fertilizer treatments were applied to 10 cultivars of durum wheat (Triticum turgidum var. durum L.) in three seasons of designed field trials. The fertilizer treatments were N0S0 ‐ neither N nor S applied; N1S0 ‐ only N applied; N1S1 ‐ both N and S applied; N0S1 ‐ only S applied. It was found, through densitometric SDS‐PAGE, that the relative proportion of different gliadin components was influenced by the distinct regimes, even though our region in the center of the Argentinean Pampa is not considered to be deficient in soil S‐availability. The effects observed were more marked in one particular season of the three studied, were principally associated with the treatment expected to produce the maximum degree of grain S‐deficiency (N1S0), and were consistent with the hypothesis that the relative content of S‐poor gliadins increases under conditions of S‐deficiency, as has been found in related crops. Multivariate reverse‐phase high‐performance liquid chromatography (RP‐HPLC) analysis of samples from the season most affected generally supported these observations, but refined them in identifying quantitative differences between all four fertilizer treatments for the cultivars studied. These findings imply that differences in industrial quality might also be expected between the fertilizer treatments. RP‐HPLC also identified numerous genetically controlled qualitative differences in the gliadins between the cultivars studied, adding to previous published work on these cultivars based on SDS‐PAGE. These observations confirmed that one particular cultivar of very low gluten strength showed a gliadin profile markedly distinct from the remaining cultivars studied.
El objetivo de este trabajo fue estudiar y evaluar la capacidad de diferentes parámetros para predecir la calidad de las harinas de trigo, analizando las relaciones existentes entre éstos y el índice de calidad industrial (ICI). Se utilizaron siete muestras de harina de trigo provistas por la CEI Barrow. Se determinaron parámetros relacionados con la calidad del grano de trigo, la molienda y la composición de las harinas. Además, se llevaron a cabo ensayos relacionados con el comportamiento de las masas (ensayo farinográfico y alveográfico) y se elaboró pan. Se calculó el ICI para cada una de las muestras y se estudió su relación con pruebas de predicción y ensayos de calidad. El ICI mostró correlaciones significativas y positivas con el contenido de macropolímero de glutenina, la extensibilidad alveográfica y el tiempo de desarrollo de la masa. Estas relaciones podrían resultar beneficiosas en la evaluación de la aptitud de las harinas para la elaboración de diferentes productos panificados, ya que constituyen determinaciones más sencillas y no requieren de equipamiento de alta tecnología ni grandes cantidades de muestra
The quality of the wheat (Triticum aestivum L.) grain is determined by several factors that lead to different physical, chemical, and/or rheological characteristics. Our aim was to evaluate the effects of the type of cultivar quality and agricultural region of production on parameters that define the commercial and industrial quality of wheat. A total of 3,486 wheat samples were collected from producer lots. The samples corresponded to commercial cultivars classified according to the quality type following a system used in Argentina which is based on the combination of commercial and baking quality indicators. These wheat varieties were produced in three different regions according to resource availability (fertility of the soil and average rainfall). The technological quality of the different grain samples and flours was determined by means of physicochemical analyses. The samples from the region of high resource availability with higher productivity and lower industrial quality differed from those from the regions of low and medium resource availability. Grain test weight and ash content were significantly affected by the interaction between the quality group of the cultivars and the region of origin. Grain protein and gluten contents were mainly conditioned by the resource availability of the region. However, the quality of the gluten was genetically conditioned by quality group of the cultivars. As results of the relationships among these parameters, most of the variables of flour quality (color, deformation energy, tenacity/extensibility ratio, and farinograph stability) would be explained by the interaction between the cultivar and the environment where it was grown.
La nueva variedad de trigo (Triticum aestivum L.) MS INTA BONAERENSE 514 (MS INTA BON 514) es una variedad de trigo rojo panadero de crecimiento primaveral, designada en la estación experimental como W08033, desarrollada y liberada por la Estación Experimental Barrow en 2014,
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