The purpose of this paper was to evaluate the production of propionic acid from the fermentation of agroindustrial effluents using a Propionibacterium acidipropionici culture. The composition of the substrates was determined by using an experimental design of mixtures, resulting in 10 trials. The substrates were fermented in batch borosilicate glass reactors at a temperature of 35°C, initial pH of 6.5, and 20 mL.L -1 of inoculum suspension. The highest yield of propionic acid production, 0.79 g of product per g of substrate, was obtained with a substrate composed only of corn steep liquor, which showed a productivity of 5.20 mg.L -1 h -1 and production of 0.40mL.L -1 . These results showed that the corn steep liquor positively influenced performance and productivity. Although the production of acid did not reach high values, the results indicate that it is possible to produce propionic acid by a biotechnological route; however, further studies are required to adapt and optimise these results.
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