Intensive agricultural practices and heavy use of inorganic fertilizers have significantly accelerated soil degradation. Mineralization of the organic matter of soil affects soil health and the abundance of soil beneficial microbes (SBMs) and its dynamics in the soil. The research to investigate the shifting of microbial population and the soil health was conducted in natural forest, agricultural, and degraded ecosystems. The research setting involved randomized block design consisting of six ecosystems, namely, natural forest, agricultural soil (oil palm: 10, 9, 8, and 7 years old), and degraded soils. The soil samples were taken with four replications from rhizomicrobiome of each land use. In the soil health analysis, it was understood that soil beneficial microbes (SBMs) consist of total bacteria, phosphate-solubilizing bacteria, nitrogen-fixing bacteria, total actinomycetes, and total fungi). The results showed that natural forest, oil palm plantation, and degraded land demonstrated a significant effect on the changes in biodiversity of SBM in rhizomicrobiome. The highest population of SBMs was in natural forest followed by the 10-year-old oil palm plantations and the lowest was recorded in degraded soils. These results confirm that the shift of forest ecosystems to agricultural soils will accelerate the degradation and decline of soil health.
The soil ecosystem plays an important role in soil health, nutrient availability, and agriculture productivity. Ecosystem alteration from natural forest into oil palm plantation has occurred in the past few years. Therefore, it is needed to study the microbial abundance which has a great role in the nutrient cycle. This experiment was conducted in August 2018 to January 2019. The experiment was arranged as randomized block design consisted of six ecosystems (natural forest, 2008 oil palm plantation, 2009 oil palm plantation, 2010 oil palm plantation, 2011 oil palm plantation, and 2018 oil palm plantation) and provided with four replications, using total plate count. The results showed that natural forest had the highest microbial communities of rhizomicrobiome (total bacteria 35.73×109, PSB 13.88×106, total actinomycetes 18.63x106, and total fungi 1.53×105)CFUg−1, followed by 2008 oil palm plantation which had the highest NFB 7.7×106 CFUg−1. 2011 oil palm plantation had the lowest microbial communities of rhizomicrobiome (total bacteria 11.06×109, PSB 2.93×106, NFB 7.76x106, total actinomycetes 2.91×106, and total fungi 0.26×105)CFUg−1. The research revealed that the alteration of natural forest to agricultural ecosystem influences the richness of microbial population in rhizomicrobiome which has a great role soil health, nutrient availability, and agriculture productivity.
AbstrakKeberhasilan aplikasi pupuk hayati berkaitan dengan viabilitas mikroba dalam pembawa, dan aplikasinya di lapangan. Penelitian ini bertujuan mengetahui viabilitas Bakteri Penambat Nitrogen (BPN) dan Bakteri Pelarut Fosfat (BPF) pada berbagai komposisi karier, konsentrasi molase, dan aplikasinya dengan berbagai dosis serta komposisi amelioran pada pembibitan kelapa sawit. Penelitian dilaksanakan dari bulan Oktober 2019 - Mei 2020 di laboratorium dan kebun percobaan Ciparanje. Tahap pertama menggunakan Rancangan Acak Lengkap dua faktor (komposisi karier dan konsentrasi molase) dengan tiga ulangan. Tahap kedua menggunakan rancangan acak kelompok faktorial dua faktor (komposisi amelioran dan dosis aplikasi) pada pembibitan kelapa sawit sebanyak tiga ulangan. Hasil riset tahap pertama menunjukkan bahwa perlakuan kombinasi karier 90% + zat aditif 10% dengan penambahan 2% molase merupakan perlakuan terbaik dengan viabilitas BPN dan BPF tertinggi sampai dengan 12 Minggu Setelah Produksi (MSP) dengan masing-masing populasi sebesar 1,43 x 108 CFU g-1 dan 1,65 x 108 CFU g-1. Viabilitas BPN maupun BPF dalam campuran carrier 90% + zat aditif 10% dengan penambahan 2% molase pada masa simpan sampai dengan 12 MSP masih memenuhi standar mutu pupuk hayati yaitu sebesar 107 CFU g-1. Hasil riset tahap kedua dosis d3 (10%) komposisi k3 (Kompos blotong 25% + Abu sawit 25% + biochar 40% + Dolomit 10%) memberikan hasil terbaik pada pertambahan tinggi batang, diameter batang dan jumlah daun.Kata Kunci: Amelioran, organik, bakteri penambat nitrogen, bakteri pelarut fosfat, karier, pembibitan kelapa sawit AbstractThe successful application of biofertilizers is related to microbial viability in the carrier, and application in the field. The research examined the viability of Nitrogen-Fixing Bacteria (NFB) and Phosphate Solubilizing Bacteria (PSB) on various carrier compositions, molasses concentrations, and field application at oil palm nursery combined with different ameliorant dosage and composition. This research was conducted from October 2019 to May 2020 at the laboratory and Ciparanje experimental garden. The first phase used a two-factor completely randomized design (carrier composition and molasses concentration) that was given three replications. The second phase used a two-factor randomized completely block design (ameliorant composition and dosage). First phase results showed that carrier mixture 90% + additive 10% with the addition of 2% molasses was the best treatment with the highest NFB and PSB viability until 12 weeks after biofertilizer production with population of about 1.43 x 108 CFU g-1 and 1.65 x 108 CFUg-1, respectively. Viability of NFB and PSB in carrier mixture 90% + additive 10% with the addition of 2% molasses at the shelf life of 12 weeks after the production still meet the biofertilizer standards in the amount of 107 CFU g-1. Ameliorant d3 dose (10%) of composition k3 (25% sugarcane boiler compost + 25% palm ash + 40% biochar + 10% dolomite) gave the best results in the increase of plant height, stem diameter and leaves number.Keywords: Ameliorant, nitrogen fixing bacteria, oil palm nursery organic carrier, phosphate solubilizing bacteria
Nitrogen fixing bacteria (NFB) plays an important role in increasing N availability for plants. Research to examine the ability of nitrogen fixing bacteria isolates to produce nitrogenase, phytohormone and the ability of nitrogen fixing bacteria isolates in the biological test process using the corn plant indicator as an indicator has been carried out from September 2018 to February 2019 in laboratories and greenhouses. The ability of nitrogen fixing bacteria was tested by the ARA method, while the phytohormone testing of nitrogen fixing bacteria was tested using the HPLC method. Bioassays using Murphy media and corn plants as indicators were performed using a randomized block design consisting of six treatments (one control and five selected NFB isolates from the selection results) and given five replications. Measurement of root length, plant height, and dry weight of plants were carried out every 2 days for 14 days. The results showed that the nitrogen fixing bacteria isolates used from North Sangattarhizobiome, East Kalimantan had different nitrogenase and differentphytohormone test results, and obtained five selected isolates based on the selection results. The results of the bioassay did not show any significant differences based on the Duncan test at the level of 5%. However, it can be seen visually the significant difference in which plants in the biological test using nitrogen fixing bacterial isolates have relatively higher plant growth and dry weight of plants than plants that are not given treatment or control.
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