Magnetic orientation of isotactic polystyrene during the
isothermal crystallization at 210
°C in the magnetic field of 6 T is studied by using the magnetic
birefringence, X-ray diffraction, and
infrared measurements. The orientation starts in the vicinity of
the end of the induction period, that is,
before the crystal growth is observed by the wide angle X-ray
diffraction This suggests that some
anisotropic structures formed in the induction period are responsible
for the magnetic orientation. Crystals
formed are aligned with the c-axis, perpendicular to the
magnetic field. Crystallization process is
accelerted under the magnetic field.
We employed £uorescence correlation spectroscopy (FCS) to analyze the characteristics of biomolecules in living cells. Protein kinase C (PKC) changes its subcellular localization from cytosol to the plasma membrane by its ligand. Using FCS, we found PKCL LI labeled with enhanced green £uorescent protein freely di¡using in cytosol. Upon 12-O-tetradecanoylphorbol-13-acetate activation, a large part of PKCL LI is anchored in the plasma membrane but some PKCL LI still moves freely near the plasma membrane. These results indicate that a di¡usion-driven transport mechanism is appropriate for the molecular mechanism of the PKCL LI localization change.
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