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Low-level laser energy has been increasingly used in the treatment of a broad range of conditions and has improved wound healing, reduced edema, and relieved pain of various etiologies. This study examined whether 635-nm low-level lasers had an effect on adipose tissue in vivo and the procedural implementation of lipoplasty/liposuction techniques. The experiment investigated the effect of 635-nm, 10-mW diode laser radiation with exclusive energy dispersing optics. Total energy values of 1.2 J/cm(2), 2.4 J/cm(2), and 3.6 J/cm(2) were applied on human adipose tissue taken from lipectomy samples of 12 healthy women. The tissue samples were irradiated for 0, 2, 4, and 6 minutes with and without tumescent solution and were studied using the protocols of transmission electron microscopy and scanning electron microscopy. Nonirradiated tissue samples were taken for reference. More than 180 images were recorded and professionally evaluated. All microscopic results showed that without laser exposure the normal adipose tissue appeared as a grape-shaped node. After 4 minutes of laser exposure, 80 percent of the fat was released from the adipose cells; at 6 minutes of laser exposure, 99 percent of the fat was released from the adipocyte. The released fat was collected in the interstitial space. Transmission electron microscopic images of the adipose tissue taken at x60,000 showed a transitory pore and complete deflation of the adipocytes. The low-level laser energy affected the adipose cell by causing a transitory pore in the cell membrane to open, which permitted the fat content to go from inside to outside the cell. The cells in the interstitial space and the capillaries remained intact. Low-level laser-assisted lipoplasty has a significant impact on the procedural implementation of lipoplasty techniques.

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In vitro effects of 635-nmdiode laser irradiation on the lipidic inclusions and the cellular fat distribution were observed in situ on a selected multilocular adipose cell in culture by an effective laser power of 3 . 1 6 mW. Selected microscopic field was 12 times sequentially irradiated, using 100 seconds exposures, a free spot of 5 mm and effective energy density of 1.6 Jcm2 per exposure. Same field was irradiated 24 times using a beam spot of 10 mm, 0.4 Jcm2. Digital microphotograph sequences permit to observe and follow changes in fat distribution. Results show changes in fat vesicles. Microscopic follow-up shows an almost empty vacuole 15 hours after irradiation, the cell was empty after 42 hours, and dies after 52 hours.

We use a tunable commercial liquid-crystal device tuned to a quarter-wave retardance to study the generation and dynamics of different types of hybrid vector beams. The standard situation where the q-plate is illuminated by a Gaussian beam is compared with other cases where the input beam is a vortex or a pure vector beam. As a result, standard hybrid vector beams but also petal-like hybrid vector beams are generated. These beams are analyzed in the near field and compared with the far field distribution, where their hybrid nature is observed as a transformation of the intensity and polarization patterns. Analytical calculations and numerical results confirm the experiments. We include an approach that provides an intuitive physical explanation of the polarization patterns in terms of mode superpositions and their transformation upon propagation based on their different Gouy phase. The tunable q-plate device presents worthy advantages, since it allows a compact and efficient generation of pure and hybrid vector beams to study these effects.

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