Grape pomace is a potential source of natural antioxidant and antimicrobial agents. Phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four Virginia‐grown grape varieties were investigated. White grape pomaces had higher (P < 0.05) solvent extraction yield than red varieties. Concentrations of total phenolic (TPC), total flavonoid (TFC), total anthocyanin (TAC), tannins, condensed tannins (CT), as well as antioxidant capacities (DPPH• and ABTS•+free radical scavenging) differed (P < 0.05) among four pomace extracts. ABTS•+ scavenging capacity was positively correlated with TPC, TFC, tannins, and CT (P < 0.05), whereas DPPH• capacity was positively correlated with TAC (P < 0.05). Nine flavonoid compounds were identified, of which catechin and epicatechin were the two most abundant. Antibacterial activity was observed against Listeria monocytogenes
ATCC 7644 and Staphylococcus aureus
ATCC 29213, but not against Escherichia coli O157:H7 ATCC 3510 and Salmonella typhimurium
ATCC 14028. L. monocytogenes was more susceptible than S. aureus.
Summary
Kabuli chickpea seeds were processed by soaking only and soaking followed either by pressure cooking or roasting. Proteins were isolated from both raw and processed seeds, and the effects of these processing approaches on the physicochemical, functional, thermal and structural properties as well as SDS‐PAGE profiles of the protein isolates were investigated. Thermal processes significantly (P < 0.05) decreased protein yield, content, colour difference, emulsifying properties and protein solubility of the protein isolates, but increased lightness and water and oil absorption capacities. Protein thermal properties, secondary structure and SDS‐PAGE banding patterns were significantly changed in thermal‐processed samples, especially those that were pressure cooked. No endothermic peak was detected in differential scanning calorimetry thermograms, and peak intensity of amide I absorption band at 1600–1700 cm−1 in Fourier transform infrared spectra reduced. The results reveal that pressure cooking had more pronounced effects on the properties of the protein isolates than other processing approaches.
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