Colorimetric methods based on the Berthelot reaction are used widely for quantitative determination of NH4‐N in biological and environmental samples. Studies to evaluate phenol and salicylate, the most commonly used chromogenic substrates, revealed minor interferences by metallic cations, whereas up to a threefold shift in absorbance was observed with 38 diverse N‐containing organic compounds. Interferences differed markedly between phenol and salicylate. The possibility of a simple correction was precluded by the fact that interferences were both positive and negative, and depended on the temperature during color development and the concentration of NH4‐N. Fourteen compounds were evaluated as alternatives to phenol and salicylate, of which the Na salt of 2‐phenylphenol (PPS) proved to be the most promising. Using PPS, macro‐ and microscale batch methods and an automated flow‐injection method were developed. These methods are simple, convenient, and sensitive. Using the PPS microscale method, for which the limit of detection is 0.17 mg NH4‐N L−1, recovery of NH4‐N added to soil extracts ranged from 98 to 104%, with a coefficient of variation of 1.4 to 2.7%. As with phenol and salicylate, precipitation of metal hydroxides was observed. Precipitation was controlled by chelation with citrate rather than ethylenediaminetetraacetic acid (EDTA), which suppressed color development by preventing monochloramine formation. Compared with Berthelot methods that use phenol or salicylate, interference by amino acids was decreased by up to 10‐fold. Interference by other organic N compounds was virtually eliminated.

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The finding of no difference in insulin sensitivity between the two groups contrasts with, but does not entirely contradict, the results of previous epidemiological studies--perhaps suggesting that longer term changes such as liver enzyme induction may be important. The difference in insulin secretion questions the validity of previous studies of the influence of alcohol on insulin sensitivity, where insulin levels were used as a surrogate for insulin resistance.

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