Conclusions and Summary
The following conclusions have been reached from the data presented: The temperature to which the serum-cell mixture was exposed had little effect upon the rate of antibody absorption. However, extreme temperatures above and below room temperature usually reduced the homologous titer with little or no effect upon the heterologous titer.The optimal absorption time was approximately 30 minutes. After this time the homologous and heterologous titers tended to level off and closely parallel each other.The optimal cell volume was dependent upon the serum used. In no case was the volume greater than 50% when the optimal dilution of serum and optimal time and temperature were used in the absorption. It is advisable to keep the cell volume to a minimum.The optimal dilution for each serum was dependent upon the complexity of the serum and the respective titers for the homologous and absorbing bloods. As a general rule, dividing the titer for the absorbing blood by 8 gives a dilution of serum satisfactory for absorption.
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