This paper shows the capability of a numerical simulation in capturing the dynamic and unsteady flow effects inside a centrifugal pump due to the impeller-volute interaction. The object of the study is a commercial centrifugal water pump with backward curved blades, which is built within a vaneless single tongue volute. For the numerical simulation, the viscous Navier-Stokes equations are handled with an unsteady calculation and the sliding mesh technique is applied to take into account the impeller-volute interaction. In keeping the unsteady terms of the equations active it is possible to correctly simulate the effects of the blade passage in front of the tongue and both the flow and pressure fluctuations induced. Time averaged numerical results are compared with the experimental performance curve and good agreement is found. The numerical flow analysis allows the study of different variables which are always difficult to measure experimentally. The dynamic variables obtained with the proposed numerical model are compared with the experimental data. In particular, the amplitude of the fluctuating pressure field at the blade passing frequency is successfully captured by the model for a wide range of operating flow rates. Therefore, the main achievement of the work is in providing the modeling possibilities for the prediction of the dynamic interaction between the flow at the impeller exit and the volute tongue. Such effects at the blade passing frequency appear to follow a clear flow rate dependent spatial pattern around the volute.

We present the task of predicting individual well-being, as measured by a life satisfaction scale, through the language people use on social media. Well-being, which encompasses much more than emotion and mood, is linked with good mental and physical health. The ability to quickly and accurately assess it can supplement multi-million dollar national surveys as well as promote whole body health. Through crowd-sourced ratings of tweets and Facebook status updates, we create message-level predictive models for multiple components of well-being. However, well-being is ultimately attributed to people, so we perform an additional evaluation at the user-level, finding that a multi-level cascaded model, using both message-level predictions and userlevel features, performs best and outperforms popular lexicon-based happiness models. Finally, we suggest that analyses of language go beyond prediction by identifying the language that characterizes well-being.

Intraneuronal accumulation of amyloid β (iAβ) has been linked to mild cognitive impairment that may precede Alzheimer's disease (AD) onset. This neuropathological trait was recently mimicked in a novel animal model of AD, the hemizygous transgenic McGill-R-Thy1-APP (Tg+/−) rat. The characterization of the behavioral phenotypes in this animal model could provide a baseline of efficacy for earlier therapeutic interventions. The aim of the present study was to undertake a longitudinal study of Aβ accumulation and a comprehensive behavioral evaluation of this transgenic rat model. We assessed exploratory activity, anxiety-related behaviors, recognition memory, working memory, spatial learning and reference memory at 3, 6, and 12 months of age. In parallel, we measured Aβ by ELISA, Western blots and semiquantitative immunohistochemistry in hippocampal samples. SDS-soluble Aβ peptide accumulated at low levels (~9 pg/mg) without differences among ages. However, Western blots showed SDS-resistant Aβ oligomers (~30 kDa) at 6 and 12 months, but not at 3 months. When compared to wild-type (WT), male Tg+/− rats exhibited a spatial reference memory deficit in the Morris Water Maze (MWM) as early as 3 months of age, which persisted at 6 and 12 months. In addition, Tg+/− rats displayed a working memory impairment in the Y-maze and higher anxiety levels in the Open Field (OF) at 6 and 12 months of age, but not at 3 months. Exploratory activity in the OF was similar to that of WT at all-time points. Spatial learning in the MWM and the recognition memory, as assessed by the Novel Object Recognition Test, were unimpaired at any time point. The data from the present study demonstrate that the hemizygous transgenic McGill-R-Thy1-APP rat has a wide array of behavioral and cognitive impairments from young adulthood to middle-age. The low Aβ burden and early emotional and cognitive deficits in this transgenic rat model supports its potential use for drug discovery purposes in early AD.

No abstract

No abstract

Experimental and numerical studies are presented on the steady and unsteady radial forces produced in a single volute vaneless centrifugal pump. Experimentally, the unsteady pressure distributions were obtained using fast response pressure transducers. These measurements were compared with equivalent numerical results from a URANS calculation, using the commercial code FLUENT. Two impellers with different outlet diameters were tested for the same volute, with radial gaps between the blade and tongue of 10.0% and 15.8% of the impeller radius, for the bigger and smaller impeller diameters, respectively. Very often, pump manufacturers apply the similarity laws to this situation, but the measured specific speeds in this case were found to be slightly different. The steady radial forces for the two impellers were calculated from both the measured average pressure field and the model over a wide range of flow rates in order to fully characterize the pump behavior. Again, a deviation from the expected values applying the similarity laws was found. The data from the pressure fluctuation measurements were processed to obtain the dynamic forces at the blade passing frequency, also over a wide range of flow rates. Afterwards, these results were used to check the predictions from the numerical simulations. For some flow rates, the bigger diameter produced higher radial forces, but this was not to be a general rule for all the operating points. This paper describes the work carried out and summarizes the experimental and the numerical results, for both radial gaps. The steady and unsteady forces at the blade passing frequency were calculated by radial integration of the pressure distributions on the shroud side of the pump volute. For the unsteady forces, the numerical model allowed a separate analysis of the terms due to the pressure pulsations and terms related to the momentum exchange in the impeller. In this way, the whole operating range of the pump was studied and analyzed to account for the static and dynamic flow effects. The unsteady forces are very important when designing the pump shaft as they can produce a fatigue collapse if they are not kept under a proper working value.

We investigated the role of adult hippocampal neurogenesis in cocaine-induced conditioned place preference (CPP) behaviour and the functional brain circuitry involved. Adult hippocampal neurogenesis was pharmacologically reduced with temozolomide (TMZ), and mice were tested for cocaine-induced CPP to study c-Fos expression in the hippocampus and in extrahippocampal addiction-related areas. Correlational and multivariate analysis revealed that, under normal conditions, the hippocampus showed widespread functional connectivity with other brain areas and strongly contributed to the functional brain module associated with CPP expression. However, the neurogenesis-reduced mice showed normal CPP acquisition but engaged an alternate brain circuit where the functional connectivity of the dentate gyrus was notably reduced and other areas (the medial prefrontal cortex, accumbens and paraventricular hypothalamic nucleus) were recruited instead of the hippocampus. A second experiment unveiled that mice acquiring the cocaine-induced CPP under neurogenesis-reduced conditions were delayed in extinguishing their drug-seeking behaviour. But if the inhibited neurons were generated after CPP acquisition, extinction was not affected but an enhanced long-term CPP retention was found, suggesting that some roles of the adult-born neurons may differ depending on whether they are generated before or after drug-contextual associations are established. Importantly, cocaine-induced reinstatement of CPP behaviour was increased in the TMZ mice, regardless of the time of neurogenesis inhibition. The results show that adult hippocampal neurogenesis sculpts the addiction-related functional brain circuits, and reduction of the adult-born hippocampal neurons increases cocaine seeking in the CPP model.

No abstract