A proposta deste estudo é apresentar uma reflexão crítica sobre dimensões que emergiram a partir de um exercício de aproximações e distanciamentos de três estruturas de pensamento utilizadas como marcos teóricos para estabelecer parâmetros curriculares na ciência escolar. Essas estruturas são os pensamentos crítico, científico e criativo. A partir destas estruturas, destacamos as aproximações, distanciamentos e, principalmente, as interações entre elas. Como resultado, encontramos sobreposições e interações entre Pensamento Científico e Crítico, Pensamento Criativo e Científico e entre Pensamento Crítico e Criativo. Estas interações devem ser evidenciadas de modo que sejam implementadas atividades intencionais e sistemáticas dirigidas à promoção do pensamento científico, crítico e criativo para a formação integral dos estudantes.
Cryopreservation protocols have been developed for eggs of Diptera, Lepidoptera, and Hymenoptera; however, for Heteroptera, such as Podisus nigrispinus Dallas, 1851 (Pentatomidae), no procedures have been described yet. The objective of this study was to evaluate the processes of dechorionation and permeabilization on the viability of eggs of P. nigrispinus with different embryonic ages. In the laboratory, embryos of 24, 48, 72, and 96 h of age were submitted to sodium hydroxide, sodium hypochlorite, isopropyl, and hexane solutions for dechorionation and permeabilization. The experiment was carried out in a 4 × 8 factorial scheme. Sodium hydroxide affects embryo viability; however, 96-h-old embryos showed higher viability when compared with 24, 48, and 72-h-old embryos. Microscope observations showed that, after the treatments, the chorion of P. nigrispinus eggs was reduced to 5.11 ± 0.30 µm. These solutions for dechorionation and permeabilization of P. nigrispinus eggs together with embryonic age affect the viability of embryos to be cryopreserved.
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