Abstract:Cryopreservation protocols have been developed for eggs of Diptera, Lepidoptera, and Hymenoptera; however, for Heteroptera, such as Podisus nigrispinus Dallas, 1851 (Pentatomidae), no procedures have been described yet. The objective of this study was to evaluate the processes of dechorionation and permeabilization on the viability of eggs of P. nigrispinus with different embryonic ages. In the laboratory, embryos of 24, 48, 72, and 96 h of age were submitted to sodium hydroxide, sodium hypochlorite, isopropyl… Show more
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