No abstract

This study spatially estimated degraded lands in Indonesia that have limited functions for food production, carbon storage, and conservation of biodiversity and native vegetation and examined their suitability to grow biodiesel species (Calophyllum inophyllum, Pongamia pinnata, and Reutealis trisperma) and biomass species (Calliandra calothyrsus and Gliricidia sepium). Results showed ~3.5 million ha of degraded lands potentially suitable for these species in Indonesia. With the all-five-species scenario, these lands had the potential to produce 1105 PJ year−1 of biomass and 3 PJ year−1 of biodiesel. With the biodiesel-only-species scenario, these lands showed the potential to produce 10 PJ year−1 of biodiesel. Despite this energy potential, however, the land sizes were too small to support economies of scale for biofuel production. The study findings contribute to identifying lands with limited functions, modeling the growth of biofuel species on regional lands, and estimating carbon stocks of restored degraded lands in Indonesia.

This study spatially estimates degraded lands in Indonesia that have limited functions for food production, carbon storage, and conservation of biodiversity and native vegetation, and examines their suitability to grow biodiesel species (Calophyllum inophyllum, Pongamia pinnata and Reutealis trisperma) and biomass species (Calliandra calothyrsus and Gliricidia sepium). Results showed that Indonesia has ∼3.5 million ha of degraded lands potentially suitable for these species. With the all-five-species scenario, these lands had the potential to produce 1105 PJ yr−1 of biomass and 3 PJ yr−1 of biodiesel. With the biodiesel-only-species scenario, these lands showed the potential to produce 10 PJ yr−1 of biodiesel. Despite this energy potential, however, the land sizes were too small to support economies of scale for biofuel production. The study findings contribute to identifying lands with limited functions, modeling biofuel-species growth on regional lands and estimating carbon stocks of restored degraded lands in Indonesia.

Legume seeds are often sown on standing rice crops a few weeks before rice harvest (relay cropping). Seeds cannot germinate in waterlogged soil under relay sowing as oxygen is depleted. However, seeds may survive under soil waterlogging if the seeds can initiate anaerobic respiration, have a large seed reserve such as carbohydrates, perform a slow water uptake during imbibition and are small in size. An example of a seed crop that can initiate anaerobic respiration is rice. The seed embryo of rice can use an alcoholic fermentation pathway from carbohydrates to produce enough energy to germinate. In legumes, seeds with a slow imbibition rate were more waterlogging tolerant than seeds with a rapid rate. This is likely due to seeds with low imbibition rates having less electrolyte leakage than seeds with a rapid imbibition rate during germination under waterlogging. A small amount of oxygen may remain on the surface of waterlogged soil. Small seeds can use the small amount of oxygen on the surface of waterlogged soil to germinate. However, large seeds often fail to use the oxygen on the surface of waterlogged soil to germinate because only a small part of large seeds remain on the surface of waterlogged soil. Therefore, small seeds are more adapted to soil waterlogging than large seeds under relay cropping. This review is focused on the physiological adaptation of legume seeds under low oxygen concentration during soil waterlogging.

Grass pea (Lathyrus sativus L.) has a Mediterranean origin and was spread to Western Europe, Africa and South Asia. Over time, this grain legume crop has become important in South Asia, where it is often affected by waterlogging at germination. Therefore, varieties with waterlogging tolerance of seeds at germination are needed. This study evaluated waterlogging tolerance in a grass pea diversity panel. First, morpho-agronomic traits of 53 grass pea genotypes from 7 diverse countries (Afghanistan, Australia, Bangladesh, Cyprus, Ethiopia, Greece and Pakistan) were measured in a glasshouse. Seeds of the collection were then sown into waterlogged soil for 6 days and is subsequently drained for 8 days. Finally, representative genotypes from each country of origin of the three survival patterns (described below) were then tested to identify the effect of seed priming on germination and seedling growth in waterlogged soil. Canonical analysis of six traits (seed weight, pod length, pod width, flowering time, time to maturity and seedling survival) showed that genotypes from Bangladesh and Ethiopia were similar. There was a significant variation amongst genotypes in waterlogging tolerance. Genotypes from Bangladesh and Ethiopia showed the highest percent seedling survival (54% and 47%), with an ability to germinate under waterlogging and then maintain growth from the first day of draining to the final sampling (Pattern 1). In contrast, genotypes from other origins either germinated during waterlogging, but did not survive during drainage (Pattern 2) or failed to germinate and had low seedling survival during waterlogging and drainage (Pattern 3). Priming seeds reduced seedling survival in grass pea. Despite Mediterranean origin, specific ecotypes of grass pea with greater waterlogging tolerance under warm wet conditions have been favoured in Bangladesh and Ethiopia where adaptation to extreme precipitation events at germination and seedling survival upon soil drainage is critical for successful crops.

Legume seeds, when relay sown following rice, may suffer from soil waterlogging and the associated hypoxia or even anoxia. This study evaluated the tolerance of grain legume species, grass pea (three genotypes), lentil (two genotypes), faba bean (two genotypes) and field pea (one genotype), to soil waterlogging in a glasshouse, to anoxia and hypoxia in temperature-controlled room at germination and seedling stages. Changes in oxygen in the surface layers of soil, with time after waterlogging, were measured by microelectrode profiling. The soil profiling showed that soil oxygen declined and then stabilised by the fourth day after waterlogging and oxygen was not detected at 8 mm below the soil surface. Germination of seeds under waterlogging for up to 12 days and seedling survival after the soil was drained for up to 36 days, were measured in pot experiments. Seed germination and/or survival in anoxia (N2-flushed solutions) and hypoxia (1.0 and 2.5 kPa oxygen) were evaluated, and so were post-anoxia or post-hypoxia recoveries, all in comparison with aerated controls. Lentil had higher seedling emergence (55%) than the other species during soil waterlogging. However, lentil had lower seedling survival (9%) than grass pea (28%) during recovery following soil drainage. Grass pea seeds were more tolerant of anoxia and of hypoxia than the seeds of the three other species. In conclusion, grass pea, with higher percent germination and seedling survival during recovery, is more tolerant to waterlogging and subsequent soil drainage than the three other grain legume species. Grass pea was also more tolerant of hypoxia and of anoxia at the seed germination stage. These findings demonstrate the superior waterlogging tolerance of grass pea in relay sowing, as compared with the other grain legumes.

Increasing the capability of nitrogen fixation in legumes is crucial because the population has been risen dramatically and predicted to be doubled by 2050. In order to feed this high population, food productivity needs to be increased. A solution to overcome this problem is through improvement of crop productivity by applying fertilizer. However, the application of fertilizer such as nitrogen is over the recommended amount and the cost is high at approximately $US 40 billion per year. Therefore, legumes are important in order to minimize the cost and enhance soil fertility through nitrogen fixation (nodulation). To achieve high nitrogen fixation, agriculture managements such as minimum tillage, breeding programs and induced mutants have been developed. In breeding program, it was found that BT-477 had high nitrogen fixation and drought tolerant based on selection among 7 common bean genotypes. Induced mutants were applied by soaking swollen seeds in EMS and resulted to higher number of nodules (10x).

PurposeThis study aims to identify the location of the micropyle, the role of the micropyle in seed germination and the association between the micropyle size and seed weight of grass peas.Design/methodology/approachFirst, the micropyle was identified by cutting the seed in half and observing the seeds under the electron microscope. Second, the micropyle was covered by lanolin to block water imbibition. The rate of imbibition and germination was then observed. Lastly, micropyle sizes of various grass pea genotypes were identified by capturing seed images under a light microscope and converting the sizes to mm2 using computer software (ImageJ).FindingsThe location of micropyle was located nearby the hilum, similar to soybean seeds. Seed imbibition was significantly lower in lanolin application (<87%) than in the control (>124%) after 24 hours of submergence. Germination was a day delay for lanolin application on the micropyle compared to lanolin application on the non-micropyle. The germination delay resulted in a significantly lower germination percentage at <57% on the micropyle lanolin application than at >79% on the non-micropyle lanolin application after 10 days of sowing. There is no correlation between the micropyle size and seed weight.Originality/valueThese findings add information on the location and the role of the micropyle for grass pea seed germination.