Comprehensive hemagglutination inhibition (HI) and neuraminidase inhibition (NI) cross reaction tests were performed using 8 of 9 serotypes of avian paramyxoviruses (PMV). The studies were designed as full scale repeating experiments which permitted an adequate statistical treatment and elaboration of quantitative criteria of antigenic kinship. The results have shown diverse antigenic relationships between different avian paramyxovirus (PMV) serotypes which were asymmetric in some cases. The antigenic relationships found by HI test did not always parallel those found by NI tests. The antigenic inter-relationships have been displayed quantitatively in a diagram. This has given a basis for some suggestions concerning: the independent antigenic drift of the HA and Nase antigenic sites of hemagglutinin-neuraminidase (HN) glycoprotein of avian PMVs; a tentative subdivision of the whole group of avian PMVs into two subgroups: the first including PMV-2 and PMV-6 serotypes and the second including PMV-1, PMV-3, PMV-4, PMV-7, PMV-8 and PMV-9 serotypes; the conception that genomic material coding for the HN glycoprotein consists of a "common-to-all-the-PMVs" portion and a "serotype-specific" portion, on one hand, and of a "conserved" portion and a "variable" portion, on the other; the ratios between the portions have been shown to be different for, at least, certain PMV serotypes; the evolutionary pathways of the avain PMV HN antigenic drift.
SUMMARYDuring surveillance of the swine population in Israel a haemagglutinating agent was isolated and identified as a paramyxovirus (PMV). Serological studies based on haemagglutination and neuraminidase inhibition (HI and NI) tests displayed a close antigenic relationship between the isolate and the prototype strain of a variety of the avian PMV serotype 3 (PMV-3). However, comprehensive HI and NI cross-reaction tests between the isolate and PMV-3 reference strains, on the one hand, and the other eight avian PMV serotypes, on the other hand, showed that the viruses compared differed in terms of their antigenic interrelationships with the other avian PMVs and also in the quantitative values of the cross-reactivity. This is interpreted to mean that the swine isolate and the avian PMV-3 prototype strain are different viruses, although very closely related, but are not different isolations of the same virus from avian and mammalian hosts.
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