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An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x 10°organisms per g (dry weight

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Induction of IL-8 secretion by cagA negative isogenic mutant of H pylori Discussion These studies with the isogenic cagA negative mutant strain of H pylori demonstrate that deletion of the CagA protein does not effect the ability of H pylori to induce IL-8 secretion from gastric epithelial cells. The CagA protein is, therefore, not functionally involved in this response but is a marker for strains with proinflammatory properties. As all of the wild-type CagA positive strains we have studied to date induce epithelial IL-8 secretion,9 lo the bacterial factor(s) stimulating this inflammatory response is clearly closely associated with the expression of cagA. The function of the CagA protein remains to be determined. The CagA protein shows considerable size variation in different strains ofHpylori, ranging from 128 to 150 kD.' Intragenic repeat sequences are the basis of this heterogeneity.' Studies with native4 and recombinant CagA