The WHO multicenter study on Listeria monocytogenes subtyping: Random amplification of polymorphic DNA (RAPD)

Wernars, K.; Audurier, A; Russell, E; Curtis, Gordon D W; Herman, Lieve; Mee-Marquet, Nathalie van der

doi:10.1016/s0168-1605(96)01146-4

Cited by 60 publications

(32 citation statements)

References 11 publications

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“…As a result, all the strains were of RAPD type II-3 ( Fig. 2) indicating that a single clone of L. monocytogenes was associated with the raw milk method is useful and reported that more studies were needed to make RAPD-typing a standard technique for general and widescale use [20]. In this study, we used a suitable primer set, D87 and MMT1, to arrange L. monocytogenes into RAPD types.…”

mentioning

confidence: 99%

Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Yoshida¹,

Takeuchi²,

Sato³

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. Twenty epidemiologically unrelated Listeria monocytogenes strains isolated from different animals, locations and on different dates in Japan were classified into 18 types by the random amplified polymorphic DNA (RAPD) fingerprinting technique with four primers. Further, seven epidemiologically related L. monocytogenes strains isolated from raw milk and a bulk tank on a dairy farm represented the same RAPD type suggesting that they were all of the same origin. Therefore, RAPD-polymerase chain reaction (PCR) analysis, which is rapid, simple and inexpensive to perform, can be used in surveys as a convenient epidemiological technique.-KEY WORDS: Listeria monocytogenes, RAPD-PCR, typing.

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mentioning

confidence: 99%

Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Yoshida¹,

Takeuchi²,

Sato³

et al. 1999

The Journal of Veterinary Medical Science

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“…RAPD analysis and some of the PCR-electrophoresis-based methods can be performed in a time frame similar to that required for -TGGE (15,21,36), making -TGGE one of the fastest typing methods. In addition, -TGGE showed minimal problems with respect to reproducibility, which can be an issue in RAPD analysis (41). Thus, the method described here appears to have improved efficiency and reproducibility compared to those of previous methods.…”

Section: Discussionmentioning

confidence: 74%

Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis

Tominaga

2006

J Clin Microbiol

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Microtemperature gradient gel electrophoresis (-TGGE) was examined for use for the rapid subtyping of Listeria monocytogenes strains. Comparison of genomes between L. monocytogenes strains F2365 and H7858 identified a sequence encoding a portion of the PRT/PTS system IIA 2 protein domain as appropriate for -TGGE analysis. Thirty-one strains belonging to 10 different serovar types were tested by PCR, and sequence analysis of the amplified products revealed that the strains comprise 11 groups. All 55 possible pairs within the 11 groups were examined by -TGGE analysis. Of these, 47 pairs could be successfully discriminated, with a total electrophoresis time of only 7 min. Moreover, Cy3/Cy5 labeling allowed rapid identification of the sequence type in unknown strains of L. monocytogenes isolated from meat. These findings collectively indicate that -TGGE can be used for the rapid analysis of L. monocytogenes strains, facilitating determination of routes of contamination when these bacteria are found in food products.Listeria monocytogenes is considered one of the most dangerous food-borne pathogens because it causes approximately 2,500 cases of listeriosis per year in the United States, with a mortality rate of ϳ20% (22). Listeriosis outbreaks have been associated with L. monocytogenes contamination of foods such as coleslaw, cheese, and milk (8). Therefore, it is critical for food producers and administrators to be able to quickly trace the route of contamination when L. monocytogenes is detected in the final food product (42). Such field tests, however, can be complicated by the existence of L. monocytogenes in the natural environment.L. monocytogenes strains comprise 13 serovars grouped into three lineages (lineages I, II, and III) (14,31,43). Among these, serovars 1/2a, 1/2b, and, especially, 4b are implicated in many cases of human listeriosis (38). Various molecular typing methods beyond serovar typing have been investigated, including ribotyping (26), pulsed-field gel electrophoresis (PFGE) (34, 44), randomly amplified polymorphic DNA (RAPD) analysis (21), PCR-electrophoresis-based methods (15, 36), microarrays (4), esterase typing (9), amplified fragment length polymorphism analysis (1, 17), and multilocus enzyme electrophoresis (11). Among these, ribotyping and PFGE have better discriminatory powers and have been widely used (16). Recently, sequence typing (ST) and its extension, multilocus sequence typing (MLST), have been applied for analysis of L. monocytogenes (5,23,29,32,33,45). These methods have the benefit of delivering relatively standardized data, allowing easy comparisons between laboratories or against databases. In addition, MLST has been shown to be superior to ribotyping and PFGE in terms of discrimination power (32, 45). Unfortunately, ST and MLST are relatively laborious and time-consuming for analyses of large numbers of samples, limiting their usefulness in larger contexts.Temperature gradient gel electrophoresis (TGGE) and denaturing gradient gel electrophoresis (DGGE) are methods for the...

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“…Over the last decade, a vast number of reports of inexpensive and rapid methods to type Listeria spp. have been published 8,20,22,62,66) . The overall goal has been to develop methods that are more discriminatory than existing serotyping and phage-typing methods.…”

Section: Genetic Properties Of L Monocytogenesmentioning

confidence: 99%

Recent Advances in the Study of the Genotypic Diversity and Ecology of Listeria monocytogenes

Kimura

2006

Microb. Environ.

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Listeria monocytogenes, an intracellular pathogen, is the causative agent of listeriosis, a serious epidemic and sporadic food-borne disease. The clinical manifestations of listeriosis include meningitis, meningoencephalitis, septicemia, spontaneous abortion, perinatal infections, and gastroenteritis. Although rare in comparison to other food-borne diseases, listeriosis has a high rate of lethality (about 30%), making L. monocytogenes an important pathogen. L. monocytogenes can survive in a broad range of ecological niches, including farm environments and food-processing plants and in a wide range of hosts, including humans and many species of mammals. Furthermore, the capacity to adapt and survive under extreme conditions allows this bacterium to exist ubiquitously in the environment and to survive and proliferate under conditions within the food supply. Although the study of L. monocytogenes has already been extensively reviewed, knowledge about this pathogen has been expanding rapidly. Against the background of the growing body of information on this bacterium, the present review mostly discusses advances made in the study of this pathogen over the last 5 years.

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The WHO multicenter study on Listeria monocytogenes subtyping: Random amplification of polymorphic DNA (RAPD)

Cited by 60 publications

References 11 publications

Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Typing Listeria monocytogenes by Random Amplified Polymorphic DNA(RAPD) Fingerprinting.

Rapid Discrimination of Listeria monocytogenes Strains by Microtemperature Gradient Gel Electrophoresis

Recent Advances in the Study of the Genotypic Diversity and Ecology of Listeria monocytogenes

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