BACKGROUND: Aflatoxin-contaminated grain consumption over the years has been known to result in serious health hazards for its consumers. The present study investigated the effects of harvest seasons and drying methods on aflatoxins in freshly harvested maize. A 2 × 3 × 3 × 3 × 3 factorial design was used; two harvesting seasons (dry and wet), maize varieties (P3966W, P4063W and P4226), moisture contents (0.15, 0.12, and 0.10 g kg −1 ), modern fabricated solar dryer (MFSD), hybrid biomass dryer (HBD) and open-air drying (OAD) methods, and packaging materials (plastic, jute and polyethylene bag) were studied, respectively. In total, 162 samples (n = 162) of maize grains (250 g each) were dried. The freshly harvested maize was shelled, dried, stored and analyzed for aflatoxins using high-performance liquid chromatography. The data obtained were subjected to statistical analysis.RESULTS: P3966W and P4063W with an initial moisture content of (0.226 and 0.234 g kg −1 ) reached a safe level of 0.10 g kg −1 using MFSD within 2-3 days, HBD within 2-3 days and OAD within 5 days. Variety P4226 with an initial moisture content of 0.228 g kg −1 reached a safe moisture level of 0.10 g kg −1 in 2, 3, and 7 days using MFSD, HBD and OAD, respectively. Aflatoxin concentration (56.00 ± 8.89 ∼g kg −1 ) was highest in P4063W at 0.15 g kg −1 moisture content, which exceeded the maximum permissible limits of 4 ∼g kg −1 recommended by the World Health Organization. CONCLUSION: Variety, type of dryer and season affect aflatoxin contamination of maize. The adoption of MFSD drastically reduced the duration of drying and consequently controlled contamination by aflatoxins.
Background Automobile repair workshops contribute immensely to the generation of soil and water contamination. This study was conducted to compare the soil microbial load, heavy metals, and consequent toxicological effects, in three (3) automobile mechanic sites. Method Soil samples were randomly collected from 3 different auto mechanic workshop in Abeokuta town of Ogun-State, Nigeria. Bacterial and fungal counts were done via standard procedures. High-performance liquid chromatography was employed for the aflatoxin quantification. Also, 24 Wistar rats were divided into 4 groups (n = 6), group 1—Control animals: orally administered distilled water, Group 2—administered soil sample solution from Ita Oshin mechanic site (I M), Group 3—administered soil sample solution from Ajebo mechanic site (A M), while Group 4—administered soil sample solution from Laderin mechanic site (L M), for two (2) weeks. Conventional methods were used to determine some physical and biochemical parameters in the rat’s serum and tissues. Results Eight bacterial and fungal genera were identified from the soil samples with Bacillus subtilis and Aspergillus niger occurring most frequently. The levels of heavy metals (lead, zinc, chromium, and cadmium) analyzed were higher than the WHO permissible heavy metal limits in all samples. The activity of liver function enzymes ALP, AST, and ALT was significantly increased in the serum of animals exposed to the 3 soil solution samples when compared with the control group, with the highest recorded at Site II. Conclusion High level of heavy metals and aflatoxins could predispose to several health-related hazards when humans are exposed to contaminated soil solutions around and within automobile mechanic areas.
Fungi constitute a major problem in the production, storage and processing of agricultural products, recent concern about the consumption of stale retail pepper in Abeokuta necessitated the need to determine the fungal population and also to quantify Aflatoxin produced by the fungi. Fungi species were isolated from 20 pepper samples (Capsicum annum) bought from different markets in Abeokuta using standard microbiological procedures and High Performance Liquid Chromatography (HPLC) was used to quantify the aflatoxin present. Isolated fungi were Aspergillus flavus (55%), Mucor sp (10%), Sacharomyces cerevisiae (20%), Aspergillus fumigatus , Rhizopus sp and Penicillium sp (5%) respectively. Results shows that fourteen of the pepper samples had no detectable aflatoxin and the aflatoxin content in the remaining six samples was 23ng/kg, 18 ng/kg, 15 ng/kg, 9 ng/kg, 6 ng/kg and 2 ng/kg,. five of these samples had aflatoxin content above the European Union maximum tolerance level of 5ng/kg. The presence of toxin producing Aspergillus flavus capable of causing food poisoning raises concern over public health risks that may be associated with the consumption of stale pepper. Keywords: Aflatoxin, Fungal contamination, Capsicum annum, stale foods, High Performance Liquid chromatography, public health
The recent increase in consumers’ preference for commercially sold bean flour necessitated the need to determine associated mycoflora and quantify the toxin production by the isolates, since several fungal genera have been associated with bean grains. Four fungal genera were isolated from fifteen beans flour samples sourced from various markets in Abeokuta by serial dilutions and pour plates methods on Potato Dextrose Agar (PDA) plates. Distinct colonies were transferred to Methyl Red Dessicated Coconut Agar (MRDCA) plates to identify toxin- producing isolates and High Performance Liquid Chromatography (HPLC) was used for aflatoxin quantification from the bean flour samples. The isolated fungal genera were, Aspergillus (60%), Fusarium (10%), Rhizopus (15%) and Mucor (5%). Nine of the fifteen samples were found positive for aflatoxigenic organisms and also produced aflatoxins that ranged from 0.006 to 0.151 ng/kg. The presence of toxin- producing organisms in the bean flour which is above the acceptable standard is a red flag that must be addressed vigorously if public health is a priority.
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