The recovery of bacterial extracellular alkaline and neutral proteinases was investigated using mineral hydrocolloid bentonite in combination with the organic flocculant Hernostan B for the removal of solid compounds and cells from fermentation broth. The flocculation was effective in the pH range of 7.0 -8.5at concentrations of bentonite of 2.0-5.0 g . dm-3 and Hernostan B of 1.0-5.0 g . d~n -~. The results show that, by means of this technique, it is possible to achieve a yield of proteinases above 90% on a laboratory, and up to 85% on a pilot plant scale.
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