Application of flocculation for extracellular proteinases recovery

Sitkey, V.; Kuzmová, E.; Horenitzký, R.; Michalík, P.; Minařík, Martin

doi:10.1002/abio.370120408

Cited by 8 publications

(1 citation statement)

References 3 publications

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“…Bentham et al (1990) and Bonnerjea et al (1988) studied protein recovery after yeast cell debris flocculation with borax followed by centrifugation and observed minimal loss to the solid floc. Sitkey et al (1992) observed 90% protein recovery after cell flocculation with organic flocculants, but studied only optimal pH. To our knowledge, a thorough study of the effect of polymer dosing conditions on protein partitioning has not been reported.…”

Section: Introductionmentioning

confidence: 80%

Extracellular enzyme loss during polyelectrolyte flocculation of cells from fermentation broth

Pearson

Heng²,

Gebert³

et al. 2004

Biotech & Bioengineering

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Association of extracellular protein product with flocculated cells reduces product yield. Here, partitioning of the enzyme subtilisin between the liquid and polyelectrolyte-flocculated and sedimented Bacillus increased as the polymer dosage was increased beyond that necessary to obtain optimum floc character (brain floc) for cell removal by centrifugation. Partitioning to the cell floc is partly physical entrapment at all polymer dosages; however, at higher levels there is also direct interaction between the polyelectrolyte and enzyme. Enzyme loss was not likely due to pH denaturation during the flocculation process because conditions were within the stable pH range of the enzyme. The direct interaction between polyelectrolyte and enzyme was characterized through turbidimetric titrations and partitioning studies. Neither changes in the polymer feed concentration nor the method of polymer addition reduced the enzyme loss at dosages optimal for cell removal.

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Section: Introductionmentioning

confidence: 80%