Sialic acid was estimated simultaneously by three methods: (A) chemical determination based on Warrerfs method (Meth. Enzymol. 6, 463-464 (1963)) with slight modifications, (B) enzymatic measurement with a commercially available test kit, and (C) high performance liquid chromatography (HPLC) according to Silver et al. (J. Chromatogr. 224, 381-388(1981)). These methods showed closely correlated (r > 0.930) results and displayed similar precision data. Interference studies demonstrated sufficient specificity for the chemical assay, which was 5 -6 times more sensitive than the enzymatic test and hence chosen for the establishment of reference values.From 249 sera from healthy people between 16 and 63 years the 0.025 -0.975-reference intervals were calculated to be 1.57-2.63 mmol/1 for 127 men, and 1.69-2.64 mmol/1 for 122 women with no significant dependence on age and sex. From 43 cerebrospinal fluids from healthy adults the respective values were 17.3 -50.4 / . These data correspond to those of the literatüre.Some chemical assays employing thiobarbituric acid were compared. They proved reliable in contrast to the reaction of serum with 4-dimethylaminobenzaldehyde.
Sialinsäure im menschlichen Serum und Liquor cerebrospinalis Methodenvergleich und ReferenzwerteZusammenfassung: Sialinsäure wurde mit drei Methoden vergleichend bestimmt: (A) Chemische Bestimmung nach Warren (Meth. Enzymol. 6, 463-464 (1963)) mit geringen Modifikationen, (B) enzymatische Messung mit einer kommerziellen Testpackung und (C) Hochleistungsflüssigkeitschromatographie (HPLC) nach Silver et al. (J. Chromatogr. 224, 381-388 (1981)). Die Ergebnisse dieser Verfahren waren eng korreliert (r > 0,930) und zeigten ähnliche Werte bei Untersuchung ' der Präzision. Störversuche ergaben eine ausreichende Spezifität für die chemische Bestimmung, die fünf-bis sechsfach empfindlicher als der enzymatische Test war und daher zur Ermittlung der 2,5-97,5%-Bereiche eingesetzt wurde. Bei der Untersuchung von 249 Seren gesunder Erwachsener zwischen 16 und 63 Jahren betrugen sie, ohne signifikante Abhängigkeit vom Alter und Geschlecht, 1,57-2,63 mmol/1 für 127 Männer und 1,69-2,64 mmol/1 für 122 Frauen. Im Liquor cerebrospinalis von 43 gesunden Erwachsenen lagen die entsprechenden Konzentrationen zwischen 17,3 und 50,4 / . Alle Werte entsprechen den Angaben in der Literatur.Wir untersuchten die Eigenschaften einiger Thiobarbitursäure-Verfahren, die im Gegensatz zur Reaktion von Serum mit 4-Dimethylaminobenzaldehyd als zuverlässig anzusehen sind.
Stenosis of the coeliac artery by compression occurs predominantly in youngish patients and three times more frequently in women than men. It gives rise to abdominal symptoms, due to chronic disturbance of intestinal arterial supply. In eight patients, operated on for this condition, the characteristic symptoms were of abdominal pain, epigastric vascular sounds, loss of weight, nausea and vomiting. Diagnosis could only be made by angiography of the coeliac and (or) mesenteric arteries, and lateral aortography. Analysis of 240 cases reported in the literature indicates that the condition is caused by compression of the coeliac artery by the medial arcuate ligament of the diaphragm, and only in a few instances by nerve tissue of the mesenteric ganglion of the coeliac plexus. Operative treatment (decompression of the artery) removes symptoms in 83% of cases.
A large nonresectable upper-abdominal tumor in a 61-year-old woman was found to have been caused by inflammatory infiltration and mechanical obstruction of the mesenteric lymph nodes resulting in obstruction to lymphatic flow. Biopsy at the root of the mesentery and of abdominal lymph nodes raised the suspicion of Whipple's disease, a diagnosis confirmed by a small-intestine biopsy. Long-term antibiotic treatment was successful. Quantitative changes in the composition of the T-cell subpopulations at the time of the diagnosis reverted to normal in the course of treatment.
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