Studies were done to derive a technique to quantify intraruminal recycling of microbial nonammonia nitrogen (NAN). After dosing 15NH4+ into the rumen, ruminal NH3N and NAN pools were sampled over time for analyses of 15N enrichment. Compartmental analysis of both pools was used to quantify the percentage of microbial NAN that recycles in the rumen. Microbial NAN does not need to be fractionated from total NAN, assuming that only microbial NAN becomes labeled with 15N and that the ratio of microbial NAN:nonmicrobial NAN remains constant over time. Based on the data obtained from eight nonlactating cattle fed 85% corn silage diets at 1.5 to 1.0% BW, percentage of intraruminal recycling of microbial NAN was related to efficiency of bacterial protein synthesis (grams of CP/100 g of OM truly digested) in a curvilinear manner (y = 100 - .073x2). From this function, recycling was predicted to be approximately 75% for dairy cows consuming 50:50 forage:concentrate diets at 3.5% of BW. However, more data are needed to evaluate intraruminal recycling of microbial NAN using this technique under different dietary conditions.

Two in vitro digestion trials were performed to determine whether the negative effect on fiber digestion when pH was maintained at > 6.2 was attributable to glucose alone or to end products of glucose fermentation. In some treatments, glucose was depleted by a previous 6-h incubation; the supernatant from this incubation was used as the buffer source for treatments using the fermented glucose medium. In trial 1, mixed cultures were grown on cellulose, soybean hulls, and corn bran in fresh media with 0 (control) or 25 mM glucose, in media previously fermented for 6 h with 0 (control) or 25 mM glucose, or in fermented control medium plus 25 mM lactic acid. The rate of NDF digestion was decreased with fermented glucose medium but not with fresh glucose medium or lactic acid medium. Concentrations of lactate, propionate, and butyrate did not appear to affect NDF digestion directly. In trial 2, six treatment media were used: control and glucose media that were either fresh or previously fermented for 6 h and fermented control and glucose media treated with a protease. Rate of NDF digestion was slower in cultures with fermented glucose medium that was treated with protease than in fermented control medium without protease. When treated with protease, rate of NDF digestion was not different between the fermented control medium and the fermented glucose medium. Thus, the negative effect on fiber digestion appeared to be attributable partially to a proteinaceous inhibitor that was produced in culture media containing a rapidly fermented sugar.

Three diets were fed to six cannulated heifers in a replicated 3 x 3 Latin square design. Diets were a high forage control, a high forage diet with dextrose (5.6% of DM), and a medium concentrate (39.7% of DM) diet. Diets contained 13.3% CP and 1% NaHCO3 and were fed as a TMR twice daily. Mean ruminal pH (6.47), glucose concentration (.55 mM), and reducing sugar concentration (.64 mM) in heifers were similar across diets. Rate of orchardgrass NDF digestion in situ was faster for heifers fed the dextrose than for those fed the medium concentrate diet, but both were similar to that for heifers fed the control diet. Heifers fed the medium concentrate and dextrose diets had faster ruminal particulate fractional passage rates than those fed the control diet. True ruminal and apparent total tract digestion of OM and NDF were similar among diets, but ruminal NDF digestion tended to be higher with the control than with the medium concentrate diet. Total NAN and bacterial N flow to the duodenum and efficiency of bacterial protein synthesis in the rumen were greater with the medium concentrate diet than with the control and dextrose diets. The results were consistent with others that demonstrated that factors related to nonstructural carbohydrates in the diet other than just low ruminal pH affect ruminal fiber digestion; however, these results were not as strong as those of our previous in vitro work.

No abstract

The productivity of cool‐season forage grasses declines during mid‐summer in the southern corn belt of the USA. Warm‐season grasses are productive during this time, but their digestibility decreases from spring to summer. The objective of this study was to investigate cell wall factors contributing to the decline in in vitrodry matter digestibility (IVDMD) of big bluestem (Andropogon gerardiiVitman) leaf blades from late spring (May) to mid‐summer (July) under three harvest regimens. Under frequent defoliation, decrease in cell wall digestibility accounted for much of the change in IVDMD. Among cell wall constituents, only xylose and alkali‐labile phenolic acids increased significantly from spring to summer. Change inp‐coumaric acid content and the ratio ofp‐coumaric acid to ferulic acid were both negatively correlated with the decrease in cell wall digestibility. While glucose and uronic acid digestibility decreased from spring to summer, xylose was consistently the least digestible of the cell wall monomers. In warm‐season grasses grown under constant environments, tiller development causes an increase in leaf structural tissue, and the data suggest that the seasonal decline in leaf blade cell wall digestibility was partially due to tiller development.