It is known that nanosized liposomes from vegetable and animal phospholipids promote survival and functional adequacy of animal cells in the course of cryopreservation. It is also known that albumine and some polysaccharides have membrane-protective properties. In this paper we investigated whether there is a synergism of cryoprotective action between soybean liposomes and macromolecular compounds: albumine, sodium alginate, methylcellulose, carageenan and hyaluronic acid. To determine the cryoprotective effect we carried out cryopreservation of bull semen using a standard procedure in the presence of the substances under investigation. Albumine and sodium alginate demonstrated the most considerable cryoprotective effect in the presence of liposomes. In addition we determined dimensional characteristics of nanoparticles in a liposome suspension without and with sodium alginate and studied the influence of autoclave treatment on the nanoparticle size and cryoprotective effect of the complex. Autoclaving caused enlargement of a small-sized fraction of nanoparticles, supposedly, sodium alginate aggregates. Besides, the autoclaving did not demonstrate any influence on the cryoprotective efficiency of the mixture.
We studied transformation of the potential anticancer drug artemisinin by the white-rot basidiomycetes Pycnoporus sanguineus and Funalia trogii. The fungi were grown on sucrose-malt broth in the presence of artemisinin for 14 days. Transformation products were extracted with ethyl acetate and analyzed by HPLC. Each of the fungi produced only a single metabolite from artemisinin. Products of transformation were identified by mass spectrometry and NMR methods. 5β-Hydroxyartemisinin was isolated from the incubation medium of P. sanguineus in 92.1% yield and 7β-hydroxyartemisinin was isolated from the incubation medium of F. trogii in 89.7% yield.
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