CITRATE-STABILIZED NANOPARTICLES OF CeO2 STIMULATE PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO

Попов, А. И.; Ермаков, А. М.; Savintseva, I. V.; Селезнева, И. И.; Полтавцева, Р. А.; Zaraisky, E. I.; Poltavtsev, A. M.; Степанов, А. А.; Иванов, В. К.; Сухих, Г. Т.

doi:10.1615/nanomechanicsscitechnolintj.v7.i3.20

Cited by 11 publications

(10 citation statements)

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“…The viability of B-50 cells incubated with microcapsules was assessed by MTT assay on the basis of the reduction of colorless tetrazolium salt (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide), as described previously. 47 Six hours after cell seeding, the medium was replaced with a medium containing 1, 10, or 100 microcapsules per cell and the viability of the cells was assessed after 24 h. Lactate Dehydrogenase (LDH) Assay. Cells were seeded in 96well plates and cultured in an atmosphere containing 5% CO 2 , at 37 °C.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

Ceria Nanoparticles-Decorated Microcapsules as a Smart Drug Delivery/Protective System: Protection of Encapsulated P. pyralis Luciferase

Попов

Popova

Gould

et al. 2018

ACS Appl. Mater. Interfaces

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The design of novel, effective drug delivery systems is one of the most promising ways to improve the treatment of socially important diseases. This article reports on an innovative approach to the production of composite microcontainers (microcapsules) bearing advanced protective functions. Cerium oxide (CeO) nanoparticles were incorporated into layer-by-layer polyelectrolyte microcapsules as a protective shell for an encapsulated enzyme (luciferase of Photinus pyralis), preventing its oxidation by hydrogen peroxide, the most abundant type of reactive oxygen species (ROS). The protective effect depends on CeO loading in the shell: at a low concentration, CeO nanoparticles only scavenge ROS, whereas a higher content leads to a decrease in access for both ROS and the substrate to the enzyme in the core. By varying the nanoparticle concentration in the microcapsule, it is possible to control the level of core shielding, from ROS filtering to complete blocking. A comprehensive analysis of microcapsules by transmission electron microscopy, scanning electron microscopy, atomic force microscopy, confocal laser scanning microscopy, and energy-dispersive X-ray spectroscopy techniques was carried out. Composite microcapsules decorated with CeO nanoparticles and encapsulated luciferase were shown to be easily taken up by rat B-50 neuronal cells; they are nontoxic and are able to protect cells from the oxidative stress induced by hydrogen peroxide. The approach demonstrated that the active protection of microencapsulated substances by CeO nanoparticles can be used in the development of new drug delivery and diagnostic systems.

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Section: ■ Experimental Sectionmentioning

confidence: 99%

Ceria Nanoparticles-Decorated Microcapsules as a Smart Drug Delivery/Protective System: Protection of Encapsulated P. pyralis Luciferase

Попов

Popova

Gould

et al. 2018

ACS Appl. Mater. Interfaces

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“…The authors have previously shown that citrate-stabilized cerium oxide nanoparticles increase the proliferation rate of human MSCs isolated from dental pulp [ 32 ] and human vartan jelly [ 33 ]. This stimulation was associated with the regulation of the redox status of DPSc and activating genes responsible for proliferative and migratory activity.…”

Section: Resultsmentioning

confidence: 99%

The Strong Protective Action of Ce3+/F− Combined Treatment on Tooth Enamel and Epithelial Cells

et al. 2022

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We studied the toxic effects of cerium and fluoride species on human dental pulp stem cells and epithelial cells of Cercopithecus aethiops as a surrogate for the human oral mucosa. The sequential use of CeCl3 and NH4F solutions in equimolar sub-toxic concentrations enabled the possible toxic effects of individual components to be avoided, ensuring the preservation of the metabolic activity of the cells due to the formation of CeF3 nanoparticles. Cerium fluoride nanoparticles and terbium-doped cerium fluoride nanoparticles exhibited neither cytotoxicity nor genotoxicity to dental pulp stem cells, even at high concentrations (10−4 M). In millimolar concentrations (from 10−5–10−6 M), these nanoparticles significantly increased the expression of genes responsible for the cell cycle, differentiation and proliferation. The formation of cerium fluoride on the surface of the mucous membrane and teeth provided protection against the development of carious lesions, periodontitis, ROS attacks and other inflammatory diseases of the oral cavity. Luminescent CeF3: Tb nanoparticles enabled the visualization of tooth enamel microcracks.

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“…Nanoceria suppressed the adipogenic differentiation of rMSCs via the suppression of ROS-induced adipogenic differentiation induction [ 169 ]. Citrate-stabilized nanoceria significantly enhanced the proliferation of MSCs in a concentration-dependent manner [ 170 ].…”

Section: Metallic Nps and Stem Cell Differentiation And Proliferatmentioning

confidence: 99%

The Impact of Metallic Nanoparticles on Stem Cell Proliferation and Differentiation

2018

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Nanotechnology has a wide range of medical and industrial applications. The impact of metallic nanoparticles (NPs) on the proliferation and differentiation of normal, cancer, and stem cells is well-studied. The preparation of NPs, along with their physicochemical properties, is related to their biological function. Interestingly, various mechanisms are implicated in metallic NP-induced cellular proliferation and differentiation, such as modulation of signaling pathways, generation of reactive oxygen species, and regulation of various transcription factors. In this review, we will shed light on the biomedical application of metallic NPs and the interaction between NPs and the cellular components. The in vitro and in vivo influence of metallic NPs on stem cell differentiation and proliferation, as well as the mechanisms behind potential toxicity, will be explored. A better understanding of the limitations related to the application of metallic NPs on stem cell proliferation and differentiation will afford clues for optimal design and preparation of metallic NPs for the modulation of stem cell functions and for clinical application in regenerative medicine.

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CITRATE-STABILIZED NANOPARTICLES OF CeO2 STIMULATE PROLIFERATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO

Cited by 11 publications

References 0 publications

Ceria Nanoparticles-Decorated Microcapsules as a Smart Drug Delivery/Protective System: Protection of Encapsulated P. pyralis Luciferase

Ceria Nanoparticles-Decorated Microcapsules as a Smart Drug Delivery/Protective System: Protection of Encapsulated P. pyralis Luciferase

The Strong Protective Action of Ce3+/F− Combined Treatment on Tooth Enamel and Epithelial Cells

The Impact of Metallic Nanoparticles on Stem Cell Proliferation and Differentiation

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