RESUMENObjetivo Determinar el efecto de la aplicación de un programa educativo con perspectiva cultural para cuidadores de personas ancianas en situación de discapacidad y pobreza en un sector de Bogotá -Colombia, sobre las variables: auto percepción de salud, funcionalidad familiar, carga del cuidador y depresión. Métodos Estudio cuasiexperimental con pre-prueba y pos-prueba. Participaron 56 cuidadores familiares, 37 en el grupo experimental (ge) y 19 en el grupo control (gc).El programa educativo aplicado al grupo experimental constaba de seis sesiones grupales con intervalo de 30 días. Cada sesión tiene cinco fases: sensibilización, información culturalmente adaptada, toma de decisiones, compromisos de acción y cierre. Fue aplicado por una enfermera experta en gerontogeriatría y enfermería transcultural. Se aplicaron las escalas de Autopercepción de Salud, Apgar familiar, Carga del cuidador y Escala de Depresión. Resultados El programa educativo con enfoque cultural tuvo efectos significativos, sobre las variables: funcionalidad familiar (T=2,506; p=0,015) y carga del cuidador (T=-2,149; p=0,036). La autopercepción en salud y depresión no presentaron diferencias significativas. Discusión Las acciones en educación en salud abordadas desde la perspectiva cultural abren una puerta a co-crear diversidad de terapéuticas derivadas del diálogo y la mediación entre los saberes populares y profesionales. Podría ser una clave frente al escaso éxito en la adherencia de los usuarios a las instituciones de salud y a los tratamientos.

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BackgroundHealthcare workers are at increased risk of infection due to occupational exposure to SARS-CoV-2-infected patients. The objective of this study was to determine the seroprevalence of SARS-CoV-2 in healthcare workers in Colombia.MethodsThis study is a cross-sectional study focused on estimating the seroprevalence of SARS-CoV-2 antibodies in healthcare workers from 65 hospitals in 10 cities in Colombia during the second semester of 2020. The seroprevalence was determined using an automated immunoassay (Abbott SARS-CoV-2 CLIA IgG). The study included a survey to establish the sociodemographic variables and the risk of infection. A multivariate model was used to evaluate the association between the results of seroprevalence and risk factors.ResultsThe global seroprevalence of antibodies against SARS-CoV-2 was 35% (95% Bayesian CI 33% to 37%). All the personnel reported the use of protective equipment. General services personnel and nurses presented the highest ratios of seroprevalence among the healthcare workers. Low socioeconomic strata have shown a strong association with seropositivity.ConclusionThis study estimates the prevalence of SARS-CoV-2 infection among healthcare workers. Even though all the personnel reported the use of protective equipment, the seroprevalence in the general services personnel and nurses was high. Also, a significant difference by cities was observed.

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A nontumorigenic cell line derived from rat hepatocytes became tumorigenic subsequent to its cultivation for 3 months in medium containing 7.5 mM DL ethionine. We used light and electron microscopy in order to observe the structural effects of this treatment. At confluence, both experimental and control cultures appeared as monolayers of contiguous polygonal cells. Subconfluent cultures of ethionine-treated cells had a minor population of elongated cells, with extended cytoplasmic processes, which were not seen in control cultures. Actin cables (visualized with a NBD-phallacidin probe), which were routinely visible in polygonal cells, were not as pronounced in elongated cells (Fig. 1). Likewise, the arrangement of microtubules (stained with FITC-antitubulin) was characteristically altered from a mainly circumferential distribution at the periphery of the cytoplasm in polygonal cells to longitudinal arrays in elongated cells.Examination by transmission electron microscopy of confluent cell cultures revealed a marked decrease in the length of desmosomal contacts between ethionine-treated cells accompanied by an increase in extracellular fibrillar material (Fig. 2).