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The inhibitive effects of newly synthesized non-ionic surfactant based on alkylene diamine surfactants on X-65 carbon steel in formation water was investigated by means of electrochemical techniques and quantum chemical study. These derivatives were characterized by FT-IR, and the surface tension and thermodynamic parameters were calculated. The polarization showed that the inhibition efficiency of the prepared compounds was increased with increasing the length of the internal alkyl chain between the two terminal amino groups of diamine. The electronic properties obtained using quantum chemical approach were correlated with the experimental inhibition efficiencies. The surface morphology of carbon steel was investigated using SEM.