Objective: The aim was to determine the botanical, GC-MS chemical characteristics and antibacterial activity of Strobilanthes. kalimantanensis as a new species. Methods: Characterization of plants through microscopic and macroscopic analysis, isolation of essential oil (EO) by steam distillation, GC-MS analysis of essential oil content and antibacterial effectiveness tests. Results: The results of the steam distillation process of essential oil produced yield 0.547%, clear and has fresh scent. GC-MS results from essential oils identified major phytochemicals, including anethole and estragole. Antibacterial activity of EO is 100 mg/ml against Staphylococcus aureus and 150 mg/ml against Bacillus subtilis, Escherichia coli, and Streptococcus epidermidis. Conclusion: Plant with the first species reported as Strobilanthes kalimantanensis contains essential oils that have antibacterial activity.