Background and Aim: Gliricidia sepium is a medium-sized leguminous plant found widely in tropical to subtropical areas. It has been used as a medicinal ingredient and in rodenticides by local communities in both Indonesia and the Philippines. This study aimed to investigate the wound healing effects of an ointment containing G. sepium leaves on inflammatory cells using a rat model. We also determined its effect on the expression of interleukin (IL) 6 and IL-1β.
Materials and Methods: We used 16 Wistar male rats aged approximately 2 months and weighing 150-200 g. They were divided into four treatment groups (T1, positive control; T2, negative control; T3, wounds treated with G. sepium from Indonesia; and T4, wounds treated with G. sepium from the Philippines), and the ointment therapies were applied to wounds for 3 days. Hematoxylin and eosin staining was performed to examine the inflammatory cells microscopically. IL-1β and IL-6 expression were observed immunohistochemically.
Results: G. sepium leaves significantly (p<0.05) decreased the number of inflammatory cells, and the expression of IL-1β and IL-6 in the group treated with Indonesian G. sepium leaves was higher than that in the group treated with G. sepium leaves from the Philippines. The leaves contain flavonoids, saponins, and tannins, which act as anti-inflammatory agents to enhance the wound healing process.
Conclusion: Our findings suggest that G. sepium leaves from both the Philippines and Indonesia possess wound healing properties.
Hypertension is an abnormality of cardiovascular condition, marked by high-blood pressure over the normal condition.
One of the factors that causes hypertension is altered equilibrium of Renin-Angiotensin-Aldosteron system (RAAS). This
condition, which commonly by genetic could causes excess production of Angiotensin II, which known as vasoconstrictor,
carried out by Angiotensin Converting Enzyme (ACE). Angiotensin II could increases the body’s blood pressure, and
stimulates the production of free radicals, causing hypertension and oxidative stress. One of the common medication for
hypertensive patient are using ACE inhibitor pills. Consumption of commercial pills, had a negative effect on the patient,
such as skin iritation, cough, allergic reaction, etc. This study was conducted to explore the potential peptide of bakasang
extract isolated from fermented skipjack tuna (Katsuwonus pelamis L.) for antihypertensive therapy, based on the activity
of protease and histopathology of cardiac tissue on hypertensive rats (Rattus norvegicus) induced by DOCA-salt. The rats
were divided into five groups: (1) negative control group, (2) hypertensive control group, (3) hypertension with captopril
therapy dose of 5 mg/kg of body weight (BW), (4) and (5) hypertension with bakasang’s peptide extract therapy dose of
200 mg/kgBW, and 300 mg/kgBW, respectively. The results showed that bakasang’s peptide extract dose of 200 mg/kgBW
significantly (p less than 0.05) decrease protease activity and improve histopathology of cardiac tissue on hypertensive rats.
Protease activity post-therapy decreased to be 39.27 % from positive hypertension group. It could be concluded that the
peptide of bakasang’s peptide extract has antihypertension effect (ACE-inhibitors), which was able to improve
histopathology and decrease protease activity of the cardiac tissue on hypertensive rats induced by DOCA-salt.
Ikan lele (Clarias sp) adalah salah satu komoditas ikan air tawar yang memiliki peminat yang tinggi. Hal ini menyebabkan para pembudidaya berusaha untuk meningkatkan usaha budidaya ikan lele. Kabupaten Tuban adalah salah satu lokasi kawasan perikanan budidaya ikan lele di Jawa Timur. Program Pengabdian Masyarakat ini bertujuan memberdayakan para petani ikan untuk memproduksi pakan mandiri, sebagai bentuk pengembangan usaha pembesaran ikan lele dalam memecahkan masalah semakin meningkatnya harga pakan ikan. Keberadaan unit produksi pakan mandiri sifatnya yang tidak memakan lahan yang luas kemudian pelaksanaan teknisnya yang praktis dan merupakan kegiatan perikanan yang berprospek ekonomis sehingga menjadi alasan untuk menjadi bagian dari kegiatan pemberdayaan masyarakat yang berbasis perikanan di Desa Campurejo, Desa Jenggolo dan Desa Tegalagung. Penerapan IPTEK mengenai pembuatan/produksi pakan secara mandiri baik berupa alat pembuat pakan dan formulasi bahan pakan itu sendiri sehingga dapat meningkatkan pengembangan usaha budidaya ikan lele yang ada di Kabupaten Tuban. Melalui program ini para anggota mitra mendapatkan pembekalan materi edukasi dalam melakukan kegiatan berbudidaya ikan lele agar di masa depan dapat mengatasi permasalahan yang terjadi dalam budidaya ikan lele sehingga produksi ikan lele meningkat dan kerugian berkurang, sehingga perekonomian anggota mitra meningkat. Berdasarkan hasil kegiatan menunjukkan bahwa target dari kegiatan Pengabdian kepada masyarakat skema Program Kemitraan Masyarakat (PKM) terpenuhi dengan adanya peningkatan ketrampilan dan pengetahuan dalam berbudidaya ikan lele yang semakin baik, peningkatan pemahaman terhadap monitoring kualitas air kolam, selain itu juga peningkatan pemahaman terhadap penyakit yang dialami ikan lele oleh para anggota mitra.Kata kunci – Ikan Lele (Clarias sp); Pakan Mandiri; Pemberdayaan Masyarakat; Manajemen Lingkungan.
Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.
Background
Graves’ disease (GD) is an autoimmune disease, and it accounts for major cases of hyperthyroidism. Antibody against thyroid‐stimulating hormone receptor/TSHR (TRAb) is responsible for hyperthyroidism and is considered as a diagnostic marker for GD. Therefore, we developed a recombinant protein of human TSHR‐169 (hTSHR‐169), which was specifically recognized TRAb in the serum of GD patients and then compare the diagnostic performance between ELISA and dot blot of TRAb tests for their ability to diagnose GD.
Methods
20 GD patients and 20 healthy individuals from the Indonesian population were enrolled. TRAb concentration and density were quantified. Comparative analysis was performed using receiver‐operating curve (ROC) analysis.
Results
For dot blot assay, the minimum concentration to detect TRAb requiring 100 ng of antigen with antiserum diluted at 1:60. For diagnosing GD, the ELISA yielded a higher AUC compared with the dot blot assay (0.95 and 0.85, respectively). Using the recommended cutoff values, the efficiency of both assays was examined by comparing the specificity and sensitivity of the assays to the clinical diagnosis. The ELISA showed 80% and 95%, while the dot blot assay showed 70% and 95% sensitivity and specificity, respectively.
Conclusion
Although the dot blot assay exhibited lower performance than the ELISA method, the dot blot assay is a simple and rapid diagnostic assay that is suitable for diagnosing GD in rural areas, in which healthcare facilities sometimes are not accessible.
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