The Potency of Pluchea indica Less as Preventive Effort to Lead (Pb) Pertubation on Rats (Rattus norvegicus) Organs

Abstract

Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.

Histopatologi Hati Tikus yang Terpapar Logam Berat Timbal

“…Pada manusia timbal diserap dari proses inhalasi sebanyak 20-50% dan dari proses menelan sebanyak 5-15% (Minarti et al, 2016). Di dalam tubuh, timbal bersifat sebagai radikal bebas (Aulanni'am et al, 2019). Seperti yang dilaporkan oleh Pratama (2020), radikal bebas merupakan molekul yang memiliki sifat tidak stabil dan reaktif, dikarenakan memiliki satu atau lebih elektron yang tidak berpasangan, dimana radikal bebas ini dapat menyerang makromolekul penting, seperti DNA, protein, karbohidrat, dan lipid sehingga dapat menyebabkan kerusakan sel dan gangguan homeostasis.…”

Local knowledge of plant-based nutrition sources from forgotten foods in Datengan Village, East Java, Indonesia

“…indica (17.36%). P. indica leaf extract can be used as therapy in counteracting free radicals and improves histopathology in the liver, jejunum, and kidney (Aulanni'am et al, 2019). The leaf extract of P. indica has anti antibacterial activities such as Enterococcus faecalis and Fusobacterium nucleatum (Pargaputri et al 2016;Pargaputri et al 2017), Propionibacterium acnes (Hafsari et al 2015), Bacillus cereus, Pseudomonas fluorescent, and Salmonella typhimurium, as well as also as an antioxidant (Srimoon and Ngiewthaisong 2015;Widyawati et al 2018).…”