Aim: This study aimed to measure the occurrence of trematodiasis in cattle along the Progo River, a district of Yogyakarta, Indonesia. The findings help to establish the magnitude of the disease and encourage prevention and treatment of this condition. Materials and Methods: Trematode eggs were extracted from 100 fecal samples collected from cattle. The eggs were examined using the sedimentation technique, and the method of Parfitt and Banks was used to differentiate Paramphistomum spp. eggs from Fasciola spp. eggs. Results: The infection rate of trematode parasites was 50%. Cattle experienced multiple infections of both Paramphistomum spp. and Fasciola spp., as well as single infections of one species or the other. All breeds were vulnerable to infections of both trematode species, although different cattle breeds, including Peranakan Ongole crossbreeds, Simmental crossbreeds, and Limousin crossbreeds, showed differences in infection rate. The highest rate of infection with Paramphistomum spp. (15.78%) occurred in the Simmental crossbreeds. The highest rate of infection (31.57%) with Fasciola spp. was in the Peranakan Ongole crossbreeds. Multiple infections of both Paramphistomum spp. and Fasciola spp. were highest in Simmental crossbreed cattle (28.97%). Conclusion: The high infection rates of trematode parasites found in fecal samples, particularly of Fasciola spp., indicate that the cattle along the Progo River in Indonesia experience a high rate of trematodiasis disease.

Penelitian ini bertujuan merancang kandidat probe dari urutan 529 bp (R529) yang terdapat 200-300 kopi dalam genom Toxoplasma gondii (T. gondii). Untuk menguji spesifitas rancangan probe digunakan perangkat lunak basic local alignment search tool (BLASTN) dari national center for biotechnology information (NCBI) dan Geniuous. Probe DNA sebesar 237 bp dilabel yang dengan digoksigenin disintesis dengan polymerase chain reaction (PCR). Kandidat probe yang dihasilkan berpotensi untuk digunakan lebih lanjut dalam deteksi keberadaan asam nukleat T. gondii pada berbagai teknik hibridisasi.