Most of the bulbous plants are known for their medicinal purposes in addition to their ornamental value. Turkey is one of the home country of many beautiful bulbous plants. In continuation of our extensive studies on finding new natural cholinesterase inhibitors from Turkish medicinal plants, Iris L. species were investigated for their in vitro cholinesterase inhibitory effects designed to assess cholinesterase inhibitor activities on both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) and antioxidant capacities with respect to their neuroprotective potential in this study. The dichloromethane and methanol extracts prepared from the bulbs of 47 Iris taxa were screened by using modified Ellmann method and the highest butyrylcholinesterase inhibitory effect was found in the methanol extract of the bulbs of Iris pseudacorus L. (Sevim, 2018). The dichloromethane sub-extract, which is obtained bioactivity-guided fractionation of methanol extract of I. pseudacorus L., was exhibited significant butyrylcholinesterase inhibitory activity (73.65 ± 2.06 %). These active sub-extract was subjected to fractionation on column chromatography and obtained six fractions. Among the fractions, coded as N5 was shown the significant butyrylcholinesterase inhibitory activity (93.78 ± 1.49 %) compared with galanthamine (80.02 ± 0.12 %). Fractionation of N5 on flash chromatography the highest butyrylcholinesterase inhibitory activity of sub-fraction coded as DS-5 was determined as 94.00 ± 1.03 %. The responsible compound from the activity of this sub-fraction was detected as irisolidone glucopyranoside based on its mass data by using LC-ESI-Q/TOF-MS-MS technique.
The genus Iris L. (Iridaceae) is a member of geophytes with attractive flowers. There are about 56 Iris taxa growing in Turkey, 24 of which are endemic. A survey of the literature indicates that the research carried out on Iris species are focused on the flavonoid and volatile compounds of the plant. In present study, the dichloromethane and methanol extracts prepared from the rhizomes of 47 Iris taxa growing in Turkey were investigated for their in vitro cholinesterase inhibitory effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) which the enzymes linked to Alzheimer's diseases and antioxidant capacities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test as well. The Iris extracts studied have been found more active against BChE than AChE. compared with 100 μg/ml galanthamine (89.29 ± 0.96 %) as reference, Iris kerneriana (coded as Y122) and Iris pseudacorus (coded as Y131) methanol extracts had significant BChE inhibition effect (respectively, 80.22 ± 1.04 % and 53.06 ± 1.13 %) at concentration of 200 μg/ml. Among tested samples, methanol extracts of I. kerneriana, I. lazica, I. pseudacorus and I. suaveolens have shown remarkable antioxidant activity at concentration of 2 mg/ml for DPPH compared with gallic acid.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.