We report the fabrication of a label free nano biosensor platform comprising single nanofiber that is derived out of multi-walled carbon nanotubes (MWCNTs) embedded SU-8 photoresist, for the detection of three important human cardiac biomarkers viz., myoglobin (Myo), cardiac Troponin I (cTn I) and Creatine Kinase-MB (CK-MB). These composite nanofibers were synthesized using electrospinning process. Single nanofibers were aligned between pairs of electrodes in-situ during the electrospinning process. The target proteins were detected using chemiresistive detection methodology. Each biomarker was detected using a specific, single, aligned nanofiber, functionalized with its corresponding monoclonal antibody. Chemiresistive detection involves measuring the change in conductance of the functionalized nanofibers upon the binding of the targeted antigen. The minimum detection limits of Myo, CK-MB and cTn I were experimentally found out to be as low as 6, 20 and 50 fg/ml respectively. No response was observed when the nanofibers were exposed to a non-specific protein, demonstrating excellent specificity to the targeted detection. These MWCNTs embedded SU-8 nanofibers based nanobiosensor platform shows great promise in the detection of cardiac markers and other proteins as they have fast response time, high sensitivity and good specificity.
Biomedical applications adapt Nano technology-based transistors as a key component in the biosensors for diagnosing life threatening diseases like Covid-19, Acute myocardial infarction (AMI), etc. The proposed work introduces a new biosensor, based on Graphene Field Effect Transistor (GFET), which is used in the diagnosis of Myoglobin (Mb) in human blood. Graphene-based biosensors are faster, more precise, stronger, and more trustworthy. A GFET is created in this study for the detection of myoglobin biomarker at various low concentrations. Because graphene is sensitive to a variety of biomarker materials, it can be employed as a gate material. When constructed Graphene FET is applied to myoglobin antigens, it has a significant response. The detection level for myoglobin is roughly 30 fg/ml, which is quite high. The electrical behavior of the GFET-based biosensor in detecting myoglobin marker is ideal for Lab-on-Chip platforms and Cardiac Point-of-Care Diagnosis.
A 2D numerical investigation has been carried out to obtain the heat transfer performance of hybrid (Al2O3 + Ag) nanofluid in a lid driven cavity over solid block under the influence of uniform as well as non-uniform magnetic field. The geometrical domain consists of a cavity containing nanofluid that is driven by means of lid moving in one direction. This circulating nanofluid will extract enormous amount of heat from the solid block underneath the cavity resulting in conjugate heat transfer. A homogenous solver based on the finite volume method with conjugate heat transfer was developed and adopted in the existing study. The heat efficient hybrid nanofluid (HyNF) pair (2.4 vol.% Ag + 0.6 vol.% Al2O3) obtained by Nimmagadda and Venkatasubbaiah [1] is used in the present investigation. Moreover, efficient non-uniform sinusoidal magnetic field identified by Nimmagadda et al. [2] is also implemented and compared with uniform magnetic field. Furthermore, the magnetic field is applied over the geometrical domain along the two axial directions separately and the effective heat transfer performance is obtained. The significant impact of extensive parameters like Reynolds number, nanoparticle type, nanoparticle concentration, magnetic field type, magnetic field location and the strength of the magnetic field on heat transfer performance are systematically analyzed and presented.
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