Biofilms grow and expand through cell differentiation into various phenotypes, which have different functions and cooperate with each other. In our experiments, we find that biofilms can heal after damaged, and we also find there is a special structure near the cut, which is called the 'Van Gogh bundles' by Jordi et al. because of its resemblance to Van Gogh's famous painting 'The Starry Night'. Here, we study the 'Van Gogh bundles' structure evolution near the cut area, and how 'Van Gogh bundles' structure facilitates the cut healing by observing microscopic images of bacterial colonies growing from wild-type and mutant strains. We find that the amount of matrix-producing cells contributes to the 'Van Gogh bundles' structure, such as curvature. Through the comparison of curvatures of 'Van Gogh bundles' and the rate of the cut healing, we find that the smaller the curvature, the faster healing rate. To better explain the above experiment observations, we establish an individual-based model and simulate the formation and growth of 'Van Gogh bundles' along the cut by giving rules for an individual cell like cell growth, division and turning rules, and also 'Van Gogh bundles' fold division rule.

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The growth discrepancy of Bacillus subtilis biofilms along different directions under the competitive growth drive the formation of anisotropic biofilm morphology directly. Two biofilms growing from two inoculating positions with different distances exhibit promoting or inhibiting growth behavior. Here we develop an optical imaging technology to observe the cell differentiation and the growth dynamics when the biofilm grows. It shows that the spatiotemporal distribution of different phenotypes affects the biofilm morphological evolution. We develop a program to calculate the velocity of cell motion within the biofilm, which is based on the feature point matching approach. We find the cell differentiation ununiformity in the neighboring region and its opposite region leads to the cell velocity difference in the competitive environment, the different cell motion further influences the biofilm morphology evolution.When biofilms grow with a long inoculating distance, there is always a gap between the them; when biofilms grow with a short inoculating distance, two biofilms gradually merge into a whole. Our work establishes a relationship between microscopic cells and macroscopic biofilm morphological which enables us to study the competitive growth process of biofilms from multiple perspectives.

Biofilms are microbial colonies that are encapsulated in the extracellular polymer secreted by cells through their proliferation and differentiation. Biofilm exists on solid surfaces, liquid surfaces or in liquid media, where the growth of bacterial biofilm is closely related to the velocity of secondary flow, the main flow and the geometry of the channel; which are hard to measure in the natural fluid environment, making the study of the biofilm streamer growth process is difficult. In this paper, we use microfluidic channels made of polydimethylsiloxane to study the growth dynamics of Bacillus subtilis biofilm streamer in flow. We observed that the biofilm streamer growth undergoes three stages with different growth characteristics: firstly, we find that the initial growth of the streamer locates at the position with the maximum value of P= Secondary flow velocity×main flow velocity. Secondly, the biofilm undergoes the floating growth around the micro column obstacle. Finally, after the transition stage, the last growth stage includes two types due to different attaching strength and mechanical properties of the biofilm. Our research provides new insights into the formation and shedding of biofilm streamer in natural and industrial environments, and helps us better understand the biofilm growth in fluid flow.