Osmotic pressure induced by extracellular matrix drives Bacillus subtilis biofilms' self-healing

Dong, Fulin; Liu, Song; Zhang, Duohuai; Zhang, Jinchang; Wang, Xiaoling; Zhao, Hui

doi:10.1016/j.compbiolchem.2022.107632

Cited by 4 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…44 Dong et al found that the osmotic pressure increased with increasing EPS content by simulating the self-healing process of Bacillus subtilis biofilms. 45 EPS are a bacterial metabolite extending from the cell surface to the aqueous solution and play a crucial role in resisting external toxic substances. 46 Covering the surface of microorganisms with EPS can change the surface characteristics of microbial aggregates, such as hydrophilicity and zeta potential, and has an impact on cell transport behavior.…”

Section: Resultsmentioning

confidence: 99%

The mechanism of sulfate on a nitrate denitrifying anaerobic methane oxidation system

Lou

Jin

et al. 2022

Environ. Sci.: Water Res. Technol.

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

The mechanism of sulfate on a nitrate denitrifying anaerobic methane oxidation system

Lou

Jin

et al. 2022

Environ. Sci.: Water Res. Technol.

View full text Add to dashboard Cite

show abstract

“…[102] Adjusting to high osmolarity incurred cellular stress Bacterial biofilms present a special osmotic environment for each participating cell, because the sugar components of the EPS generates osmotic pressure and affect water distribution, retention and the ability of the biofilm to repair structural disruptions. [45,[103][104][105][106][107] The senorkinase KinD (Figure 1C) is involved in detecting matrix components, and as a result of its signaling activity negatively affects matrix gene expression at high osmolarity. [103] Furthermore, the air-exposed surface of the B. subtilis biofilm is covered by the hydrophobin BslA, thereby endowing it with Teflon-like attributes (Figure 1B).…”

Section: Roles Of Rema In Nitrogen Metabolismmentioning

confidence: 99%

The many faces of the unusual biofilm activator RemA

et al. 2022

View full text Add to dashboard Cite

Biofilms can be viewed as tissue‐like structures in which microorganisms are organized in a spatial and functional sophisticated manner. Biofilm formation requires the orchestration of a highly integrated network of regulatory proteins to establish cell differentiation and production of a complex extracellular matrix. Here, we discuss the role of the essential Bacillus subtilis biofilm activator RemA. Despite intense research on biofilms, RemA is a largely underappreciated regulatory protein. RemA forms donut‐shaped octamers with the potential to assemble into dimeric superstructures. The presumed DNA‐binding mode suggests that RemA organizes its target DNA into nucleosome‐like structures, which are the basis for its role as transcriptional activator. We discuss how RemA affects gene expression in the context of biofilm formation, and its regulatory interplay with established components of the biofilm regulatory network, such as SinR, SinI, SlrR, and SlrA. We emphasize the additional role of RemA played in nitrogen metabolism and osmotic‐stress adjustment.

show abstract

“…Matrix producing cells are able to transport a mixture of proteins and extracellular polysaccharides (EPS), which tightly encloses the cell populations in the bio lm. The research shows that EPS in the bio lm increases the difference in the concentration of macromolecules with that in agar matrix, and the resulting osmotic pressure promotes the transportation of nutrients in the bio lm, which is conducive to drives surface expansion [22,23] and self-healing of the bio lm [24].…”

Section: Energic Analysis Of Bio Lm Growth In Pattern IVmentioning

confidence: 99%

Analysis of biofilm expansion rate on agar substrates with different stiffness under limited nutrition

Li²,

Kong³

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

In this study, we explore the growth of Bacillus subtilis biofilm on substrates with different agar concentrations, and obtain the area, morphology information and fluorescence intensity of biofilms by using optical imaging technologies. We find that expansion rate variations of biofilms growing on substrates with high and low agar concentration (1.5wt%, 2.0wt%,2.5wt%) are not in the same phase. In the first three days’ growth, the interaction stress between biofilm and each agar substrate increases, which makes the biofilm expansion rate decreases before wrinkle pattern IV (branches) comes up. After three days, in the later growth stage after wrinkle pattern IV appears, the biofilm has larger expansion rate growing on 2.0wt% agar concentration, which has the larger wrinkle distance in wrinkle pattern IV reducing energy consumption. The above findings are further verified through images of the fluorescence intensity of matrix producing cells of each biofilm. Our study shows that the stiff substrate does not always inhibit the biofilm expansion, although it does in the earlier stage, after that, mature biofilms acquire larger expansion rate by adjusting the growth mode through the wrinkle evolution even in nutrient extremely depletion.

show abstract

Osmotic pressure induced by extracellular matrix drives Bacillus subtilis biofilms' self-healing

Cited by 4 publications

References 25 publications

The mechanism of sulfate on a nitrate denitrifying anaerobic methane oxidation system

The mechanism of sulfate on a nitrate denitrifying anaerobic methane oxidation system

The many faces of the unusual biofilm activator RemA

Analysis of biofilm expansion rate on agar substrates with different stiffness under limited nutrition

Contact Info

Product

Resources

About