Lipase-catalyzed ethanolysis of triolein was studied as a model for biodiesel production. Four lipases were immobilized on porous polypropylene, and ethanolysis reactions were carried out in methyl t-butyl ether. The reaction products were analyzed using gas chromatography. Three of the four lipases studied were efficient in the conversion of triolein to 2-monoolein, but slow in the final step of producing glycerol. However, Candida antarctica lipase B was slow in the conversion of triolein, but more efficient in the subsequent two steps than the other lipases. The 1,3-selectivity of the lipases was less pronounced for the monooleins than for triolein. Silica gel was investigated as a catalyst for acyl migration, showing an increase in biodiesel yield with three of the lipases, but a reduction in yield when C. antarctica lipase B was used. The highest biodiesel yield (96 %) was obtained with a combination of Rhizopus arrhizus lipase and C. antarctica lipase B.Electronic supplementary materialThe online version of this article (doi:10.1007/s11746-014-2465-7) contains supplementary material, which is available to authorized users.
Transesterification of linseed oil and pork lard mixture with ethanol was studied by applying a biotechnological method. The effectiveness of 16 lipases was evaluated, and the most effective lipase, Lipolase 100 L (EX), was selected for reaction condition optimisation by response surface methodology based on a Box-Behnken design. The influence of the main process parameters was evaluated, including the reaction temperature, ethanol to triglyceride molar ratio, lipase amount, and reaction time on transesterification yield. The optimal reaction conditions were found as follows: reaction temperature of 32.79 C, ethanol to oil molar ratio of 5.99, lipase concentration of 6.39%, and reaction time of 7.95 h, with an expected transesterification yield of 52.2%. Optimised enzymatic pork fat and linseed oil mixture transesterification with ethanol was less effective compared with the chemical transesterifications described elsewhere; therefore, the step-wise transesterification procedure should be applied by adding ethanol and percent preparation in the reaction mixture after glycerol phase separation. V C 2015 AIP Publishing LLC.
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