Optimisation of enzymatic transesterification of linseed oil and pork lard mixture with ethanol using response surface methodology

Abstract

Transesterification of linseed oil and pork lard mixture with ethanol was studied by applying a biotechnological method. The effectiveness of 16 lipases was evaluated, and the most effective lipase, Lipolase 100 L (EX), was selected for reaction condition optimisation by response surface methodology based on a Box-Behnken design. The influence of the main process parameters was evaluated, including the reaction temperature, ethanol to triglyceride molar ratio, lipase amount, and reaction time on transesterification yield. The optimal reaction conditions were found as follows: reaction temperature of 32.79 C, ethanol to oil molar ratio of 5.99, lipase concentration of 6.39%, and reaction time of 7.95 h, with an expected transesterification yield of 52.2%. Optimised enzymatic pork fat and linseed oil mixture transesterification with ethanol was less effective compared with the chemical transesterifications described elsewhere; therefore, the step-wise transesterification procedure should be applied by adding ethanol and percent preparation in the reaction mixture after glycerol phase separation. V C 2015 AIP Publishing LLC.

Optimization of lipase‐catalyzed polymerization of benzyl malolactonate through a design of experiment approach

“…DoE technique has been successfully used more than 20 years ago to assess the influence of various polymerization parameters on the characteristics of the resulting poly(D,L‐lactide) . Latter, some groups have optimized lipase‐catalyzed esterification and transesterification using experimental design methods …”