Adiponectin (AdipoQ) and its receptors (AdipoRs) are strongly related to growth and development of skeletal muscle, as well as glucose and lipid metabolism in vertebrates. Herein we report the identification of the first full-length cDNA encoding an AdipoR homolog (Liv-AdipoR) from the decapod crustacean Litopenaeus vannamei using a combination of next generation sequencing (NGS) technology and bioinformatics analysis. The full-length Liv-AdipoR (1,245 bp) encoded a protein that exhibited the canonical seven transmembrane domains (7TMs) and the inversed topology that characterize members of the progestin and adipoQ receptor (PAQR) family. Based on the obtained sequence information, only a single orthologous AdipoR gene appears to exist in arthropods, whereas two paralogs, AdipoR1 and AdipoR2, have evolved in vertebrates. Transcriptional analysis suggested that the single Liv-AdipoR gene appears to serve the functions of two mammalian AdipoRs. At 72 h after injection of 50 pmol Liv-AdipoR dsRNA (340 bp) into L. vannamei thoracic muscle and deep abdominal muscle, transcription levels of Liv-AdipoR decreased by 93% and 97%, respectively. This confirmed optimal conditions for RNAi of Liv-AdipoR. Knockdown of Liv-AdipoR resulted in significant changes in the plasma levels of ammonia, 3-methylhistine, and ornithine, but not plasma glucose, suggesting that that Liv-AdipoR is important for maintaining muscle fibers. The chronic effect of Liv-AdipoR dsRNA injection was increased mortality. Transcriptomic analysis showed that 804 contigs were upregulated and 212 contigs were downregulated by the knockdown of Liv-AdipoR in deep abdominal muscle. The significantly upregulated genes were categorized as four main functional groups: RNA-editing and transcriptional regulators, molecular chaperones, metabolic regulators, and channel proteins.
To investigate the catches of jack mackerel fishery, a series of fishing experiments was conducted in the high seas of the southeastern Pacific Ocean (30°S-38°S, 82°W-95°W) during the period of 9 August to 18 December, 2003 by commercial fishing vessel and research vessel. The number of 205 tows fishing was carried out in the southeastern Pacific Ocean. The total catch was 1,988 ton and CPUE was 2.4 ton/hour. CPUE showed high values in the frontal zone during the survey. Main target species caught from the experimental fishing were the jack mackerel, Trachurus murphyi (98.1%) and chub mackerel, Scomber japonicus (1.9%) as bycatch. Body length of the jack mackerel was different between female and male. The high mean catch per unit effort of jack mackerel was showed when the fishing ground of jack mackerel fishery was over the 110°W in the southeastern Pacific Ocean. But the fluctuation of the catch per unit effort in the western part of fishing ground was not matched with those year. Reliable physical and oceanographical information will be useful for the efficiency of fishing activity. According to the result of monthly movement of center of fishing ground, the fishing activity of jack mackerel fishery was performed northward in the southeastern Pacific Ocean as time passes.
To investigate the catches of squid jigging fishery, a series of fishing experiments was conducted in the high seas of the Northwest Pacific(40 43 N, 150 155 E) during the period of 1 August to 22 October 2005 by commercial fishing vessel. The number of 142 test fishing was carried out in the Northwest Pacific during 83days. The total catch were 47,524kg as 4 squid species and CPUE was 8.9kg/line day. CPUE showed high values in the frontal zone during the survey. Main squid species caught from the experimental fishing were the neon flying squid, Ommastrephes bartrami(96.8%) and the boreopacific gonate squid, Gonatopsis borealis(3.2%). Dorsal mantle length of the neon flying squid were increased by the time and increasing of the hook size. Loss rate of the neon flying squid in the water showed the highest values in 28.2% compare to the others. And the loss rate of the front roller and in the air were 1.1% and 1.0%, respectively.
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