Introduction: Extensive human and animal research shows that exercise has beneficial effects on several clinical outcomes in patients suffering from multiple sclerosis (MS). The study aimed to address the effect of aerobic training with the consumption of probiotic on the myelination of nerve fibers in a cuprizone-induced demyelination mouse model of MS. Methods: Mice, which were exposed to cuprizone (CPZ) for 13 weeks, were subjected to motor and balance tests in week 5. They (n = 5 per group) were assigned to five groups of control (C), MS, MS with exercise (MS + Exe), MS with probiotic (MS + Pro), and MS with probiotic and exercise (MS + Pro + Exe) randomly. The exercise groups carried out aerobic exercises 5 days/week for 2 months. The mice received probiotic by gavage. Performance and balance tests were repeated when the eight-week protocol of exercise and probiotic consumption was finished. One day after these interventions, they were sacrificed for biochemical and molecular biology analyses. Results: The results showed that myelin basic protein (MBP) was increased in the MS + Pro + Exe, MS + Pro, and MS + Exe compared to the MS group (P<0.05). The mRNA of nestin showed an increase in MS + Pro + Exe, MS + Exe, and MS + Pro groups compared to the MS group, but this increase in MS + Pro + Exe and MS + Exe groups was not significant compared to the control and MS groups (P>0.05). Conclusion: According to the results, lifestyle interventions can be effective against demyelinating-inflammatory processes that happen in the brains of MS patients.
Objective: Multiple sclerosis (MS) is a central nervous system disorder characterized by inflammation, demyelination and neurodegeneration. It has been suggested that exercise and intake of probiotics might influence MS pathology and, thereby, slow down the disease process in MS patients. Therefore, this study aimed to investigate the effect of exercise training with Lactobacillus plantarum intake on ionized calciumbinding adaptor molecule 1, glial fibrillary acidic protein, interleukin (IL)-6 and IL-1β in cuprizone-induced demyelination mouse model of MS. Methods: Mice were exposed to cuprizone for 13 weeks. In the fifth week, motor and balance tests were carried out on them. The mice (n = 5 per group) were randomly divided into five groups: control (C), MS, MS with exercise (MS + Exe), MS with probiotics (MS + Pro) and MS with probiotics and exercise (MS + Pro + Exe). The exercise groups carried out aerobic exercises 5 days a week for 2 months. The mice received probiotic by gavage. One day after the interventions finished, the mice were killed. Biochemical and molecular biology analyses were carried out. Results: Ionized calcium-binding adaptor molecule 1 and glial fibrillary acidic protein expression values in the MS + Pro + Exe, MS + Pro, and MS + Exe groups showed a significant decrease compared with the MS group (P < .05). Also, glial fibrillary acidic protein gene expression in the intervention groups showed an insignificant decrease (P > .05). The IL-6 and IL-1β values in the MS + Exe and MS + Pro + Exe groups showed a significant decrease compared with the MS group (P < .05). Conclusions: Exercise with the intake of probiotics improved demyelination in the cuprizone-induced demyelination mouse model of MS.
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