The aim of this study was to evaluate the effect of oodev on the gonadal maturation and characteristic of spermatophore and spermatozoa produced by the wild male black tiger shrimp against eyestalk ablation. The treatments were two doses of oodev injection at 0.5 (OD0.5) and 1 (OD1.0) mL/kg of body weight and a control was eyestalk ablation (AB). The male stock of tiger shrimp used was from wild with body weight ranged from 55-85 g, stocked into three of 10 tonnages concrete tanks with density of 25 males/tank. Oodev injection was applied for two times with one week interval. Tiger shrimp of OD0.5 group produced the highest number of gonadal maturing which was 84% followed by AB (68%) and OD1.0 (64%). Oodev injection was able to shorten the time required for spermatophore maturation at maturation phase which happened simultaneously within 7 days post injection compared to that of ablated males. Positive correlation (R2=0.612) was detected between the shrimp weight and weight of spermatophore of the tiger shrimp while correlation between spermatophore weight and number of spermatozoa was relatively low (R2=0.415). Total fatty acid tented to be higher in males injected with oodev compared to ablated males. Concentration of arachidonic acid in the muscle of male stock in OD1.0 group was extremely low of 0.0037% of total lipid compared to AB (0.3190%) and OD0.5 (0.2806%). Oodev injection at the dose of 0.5 mL/kg of tiger shrimp could improve the number of males stock producing spermatophore compared to eyestalk ablation. Simultaneously matured-spermatophore of wild male tiger shrimp within short time could be achieved through oodev injection.Keywords: oodev, spermatophore, reproduction, tiger shrimp ABSTRAK Tujuan penelitian ini adalah mengevaluasi efek hormon oodev terhadap pematangan gonad dan karakter spermatofor dan spermatozoa yang dihasilkan oleh induk udang windu jantan alam dibandingkan dengan teknik ablasi tangkai mata. Perlakuan yang dicobakan adalah dua dosis injeksi oodev yaitu 0,5 (OD0,5) dan 1 (OD1,0) mL/kg bobot tubuh dan kontrol yaitu ablasi tangkai mata (AB). Induk udang windu jantan yang digunakan berasal dari alam berbobot antara 55-85 g, ditebar dalam tiga bak pematangan gonad berkapasitas 10 ton dengan kepadatan 25 ekor/ bak. Injeksi oodev dilakukan dua kali dengan interval satu minggu. Induk udang windu pada OD0,5 menghasilkan jumlah induk matang gonad tertinggi yaitu 84%, diikuti oleh AB (68%) dan OD1 (64%). Injeksi oodev mampu mempersingkat masa pematangan spermatofor pada fase maturasi dan pematangan tersebut terjadi secara simultan setelah 7 hari pascainjeksi dibandingkan dengan induk yang di ablasi. Korelasi positif (R2=0,612) dideteksi antara bobot tubuh dan bobot spermatofor induk udang windu, sedangkan korelasi antara bobot spermatofor dan jumlah spermatozoa relatif lemah (R2=0,415). Total asam lemak daging cenderung lebih besar pada induk udang windu jantan yang diinjeksi oodev dibandingkan yang diablasi. Konsentrasi arachidonic acid dalam daging induk yang diinjeksi OD1 sangat...
A purse seine is a fishing tool made of nets which are operated by circling the hordes of fish to a bowl-shaped tool at the end of the fishing process and is commonly used to catch pelagic fish by fishermen at UPT PPP Pondokdadap. The purpose of this study was to determine the technical aspects and income of the purse seine vessels in Sendang Biru village and to analyze the feasibility of the purse seine fishery business in the Sendang Biru village. This research was conducted from May to June 2019 at UPT PPP Pondokdadap, Sendang Biru, Malang Regency. The research sample was taken by purposive sampling. Data analysis using descriptive analysis and business financial analysis. The results of the calculation of the business analysis and investment on the purse seine ship found a profit of IDR 1,394,798,872, R/C of 43, PP of 13 months, ROI of 92, NPV of IDR 90,337,780,072, IRR of 633.8 %, and Net B/C is 61. The results show that the purse seine fishery business meets the requirements and is still feasible to be implemented.
Bakteri selulolitik dan amilolitik mampu mengubah selulosa dan amilum menjadi glukosa serta mampu menghasilkan enzim selulase dan amilase. Bakteri selulolitik dan amilolitik diisolasi dari rumen sapi. Tujuan dari penelitian ini adalah untuk mendapatkan bakteri selulolitik dan amilolitik dari rumen sapi yang memiliki nilai indeks selulolitik dan amilolitik yang tinggi, serta mampu memenuhi syarat untuk dijadikan sebagai probiotik. Screening bakteri pada rumen sapi menghasilkan enam isolat yaitu AR, BR, CR, DR, ER, dan FR. Uji aktivitas selulolitik dilakukan pada substrat carboxy methyl cellulose (CMC) dan amilolitik pada amilum, uji ketahanan terhadap kondisi asam (pH 3), pengamatan pertumbuhan bakteri selama 30 jam, uji antagonistik terhadap bakteri patogen Aeromonas hydrophila, uji penempelan bakteri dan uji patogenisitas bakteri pada ikan. Hasil penelitian menunjukkan bahwa aktivitas selulolitik terbesar pada isolat AR (2,67 ± 0,35 cm) dan aktivitas amilolitik terbesar pada isolat AR (4 ± 0,60 cm). Hanya empat isolat (AR, BR, ER, dan FR) yang dilakukan uji lanjut. Keempat isolat mampu bertahan dalam kondisi asam pH 3 selama delapan jam dengan nilai OD terbesar pada isolat ER (1.137). Uji antagonistik menunjukkan bahwa isolat AR, ER, dan FR mampu menghambat pertumbuhan bakteri Aeromonas hydrophila. Isolat FR memiliki nilai antagonistik terbesar yang ditandai dengan terbentuknya zona hambat 17 mm. Uji penempelan bakteri untuk membuktikan bahwa isolat mampu menempel pada usus ikan. Uji patogenesitas pada isolat yang didapatkan guna membuktikan bahwa isolat tidak bersifat patogen terhadap inang. Berdasarkan hasil penelitian dapat disimpulkan bahwa isolat yang didapatkan dari rumen sapi termasuk bakteri selulolitik dan amilolitik yang telah memenuhi syarat sebagai kandidat probiotik pada ikan.Cellulolytic and amylolytic bacteria can transform cellulose and starch into glucose and produce the cellulase and amylase enzymes. These types of bacteria can be found in and isolated from cow’s rumen. Thus, the purpose of this study was to obtain potential cellulolytic and amylolytic bacteria from cow’s rumen with a high cellulolytic and amylolytic index value and can be qualified as probiotics. The screening of bacteria in the cow’s rumen produces six isolates i.e. AR, BR, CR, DR, ER, and FR. The parameters observed were: the cellulolytic activity in carboxymethyl cellulose (CMC) substrate, amylolytic activity on starch substrates, resistance test to acidic conditions (pH 3), bacterial growth for 30 hours, antagonistic to pathogenicity test to bacteria Aeromonas hydrophila, bacterial attachment test, and bacterial pathogenicity test in fish. The results showed that the largest cellulolytic and amylolytic activities were observed in AR isolates with a value of 2.67 ± 0.35 cm) and 4 ± 0.60 cm, respectively. Only four isolates (AR, BR, ER, and FR) were used in further tests. The four isolates were able to survive in the acidic conditions of pH 3 for 8 hours with the largest (OD) value was achieved by ER isolates (1,137). The growth of each isolate was different. The antagonistic test showed that the three isolates could inhibit the growth of A. hydrophila. FR isolates had the greatest antagonistic values characterized by the formation of an inhibition zone of 17 mm. Bacteria attachment test proved that the isolates were able to stick in the fish gut. The pathogenicity tests also proved that the isolates were not pathogenic to the host.
Betta splendens adalah salah satu jenis ikan hias yang mempunyai daya tarik dan nilai ekonomis pada kualitas warna yang dimunculkan dari tubuhnya. Tujuan dari penelitian ini adalah untuk mengetahui pemberian pakan hidup yang diperkaya dengan astaxanthin Carophyll© red dapat meningkatkan kualitas warna merah pada ikan cupang halfmoon merah serta untuk mengetahui dosis Carophyll© red yang memberikan nilai optimal bagi warna ikan cupang. Metode penelitian yang digunakan adalah menggunakan metode rancangan acak lengkap (RAL) dengan perlakuan dosis Carophyll© red yang diperkaya melalui Daphnia sp. dengan 5 perlakuan 5 ulangan : 0 g (kontrol); 0,25 g L-1; 0,5 g L-1; 0,75 g L-1 dan 1 g L-1. Ikan yang digunakan adalah ikan cupang halfmoon dengan warna yang seragam. Pengamatan yang dilakukan selama 28 hari meliputi kualitas warna yang dilakukan dengan menggunakan alat color reader, perhitungan jumlah konsumsi Daphnia sp. selama pemeliharaan, pertumbuhan panjang, bobot yang dihasilkan dan kualitas air. Hasil penelitian menunjukkan bahwa peningkatan kualitas warna pada ikan cupang optimal dengan dosis 0,25-0,5 g L-1. Namun peningkatan warna bersifat temporer. Tidak terdapat perbedaan signifikan terhadap pertumbuhan bobot dan panjang mutlak, laju pertumbuhan berat mutlak. Pengkayaan Dapnia sp. dengan Carophyll© red dapat mingkatkan konsumsi pakan dan sintasan ikan Cupang.
Purpose the research to know influence giving leaf durian infusedto anesthesia process gurami fish with weight 100-150 gr usepredetermined dosage by upper threshold test and lowerthreshold test. Then, use formula EC-100 (EffektivityConsentrastion) to know fainting time fish and conscious timefish. On threshold test use among others concentrate 1000ppm,2500ppm, 5000ppm and 7500ppm. That at concentration7500ppm is upper threshold because gurami fish die by soakingleaf Durian infused for 24 hours while at concentration 5000pmis lower threshold because gurami fish not die by soaking leafDurian infused for 48 hours. Next to search concentration rowwith use formula EC-100 and than available concentrate5400ppm, 5900ppm, 6400ppm, 6900ppm and 7400ppm. Theconcentration be used to research treatment, P1:5400ppm,P2:5900ppm, P3:6400ppm, P4:6900ppm and P5:7400ppm. TheMethod of this research is a complete random design (RAL) usefive treatments and three replications. The main parameterobserved lamely fainting time fish and conscious time fish. Theresult fainting time fish (minute) on P1: 200, 67 P2: 198,33 P3:196 P4: 193 and P5: 186,67 while results conscious time fish(minute) on P1: 22 P2: 22,67 P3: 23,33 P4: 28,33 and P5: 30,33.Next statistic test use method ANOVA and BNT test with level 5%indicate significantly different, and 1 % indicate very differentlyreal. The obtainable conclusion that research use leaf durianinfusum to anesthesia process gurami fish very differently real tofainting time and conscious time. The best treatment on P5(7400ppm) because gurami fish fast to fainting time and long afor conscious time.Keywords: Anesthesia; Durian Leaf; Gouramy; Infused; Saponin
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