A new cell line, PPC-I, has been established from a specimen obtained from a patient with a poorly differentiated adenocarcinoma of the prostate. This is the first line of its type derived from a primary prostatic tumor site. PPC-I cells have become immortalized in culture, exhibit transformation parameters including relaxed growth factor requirements and anchorage-independent growth, and are highly tumorigenic in nude mice. Cytogenetic studies by G-banding revealed a grossly abnormal karyotype, with a modal chromosome number of 84, multiple marker chromosomes including both homogeneously staining regions and double minutes and clonal loss of chromosomes 3, 5, 10, 15 and Y.
320 0 1 ' I I I 3 ? 14 23 31 OAYS AFTER INlECnONS F I G . 3. Potentiation of a commercial aqueous testosteroae suspension by aluminum phosphate. Seminal vesicle response of immature rats to a single intramuscular injection.slower release from the depot site, it follows that a depot of micro-crystalline testosterone, subcutaneously or intramuscularly, should be ideal for slow absorption. As yet, there is no experimental demonstration of the mechanism by which aqueous A1P04 suspmsion of testosterone elicits a greater androgenic respmse than the aqueous suspensions without Alp&.Summary. The experimental data confirm the depot effect described by Carlinfanti and his co-workers following subcutaneous injection of ATAP suspensions in the guinea pig.The effect also has been demonstrated in immature castrate rats after intramuscular injection. In both instances marked increase in response and duration of biological activity were obtained. Potency ratios and indices of duration of action indicate that each milligram of testosterone in aqueous aluminum phosphate suspension is ( 1 ) two or more times as active, and (2) acts approximately two to three times as long as the same quantity of testosterone in aqueous suspension alone.
Human prostate tissue gave rise to essentially pure cultures of basal cells in vitro. Careful analysis by phase-contrast microscopy and photography suggested that these cells attempt to differentiate into secretory acinar cells. This was confirmed by immunocytochemical analysis for prekeratin which is present only in basal cells in situ and for prostatic acid phosphatase, prostate-specific antigen, and prostate fluid antigen, which are present only in secretory acinar cells in situ.
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