The biological/pharmacological activities of any herbal drug are determined by the bioactive components contained in the plant(s) used in its formulation, these substances are greatly affected by the quality control system adopted during and after production processes and handling of such product. Thin Layer Chromatography is one of such quality control parameters that demonstrate uniqueness and uniformity between various substances, thus serving as an identity for such products. Pax Herbal Malatreat tea is a polyherbal drug used in the management and treatment of malaria symptoms. This study evaluated the different phytoconstituents present and developed fingerprint profiles (TLC) for the drug (Pax Herbal Malatreat Tea) to serve as a quality control check during the production consistency and market uniqueness. Qualitative phytochemical and chromatographic analyses were carried out using standard methods. The qualitative test revealed the presence of Tannin, Flavonoid, Saponin, Steroids, Reducing sugar, and Terpenoids, and the finger-print chromatograms after development with chloroform-ethyl acetate (9.5:05) showed five distinct components at 365 nm and four spots when sprayed with 20% methanolic sulphuric acid and viewed under visible light. From this present report, an identity card has been developed for the drug (Pax Herbal Malatreat Tea) via bioactive composition and TLC profiles which can be used in accessing the quality and consistency of the product.
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