SYNOPSISIt is known that dichloroethylamines in which the two chloroethyl groups are bound to different N atoms at a given distance from each other retain their activity and considerably lower their toxicity. It was assumed that by controlling the distance between the two chloroethyl substituents to the polymer chain a product of analogous action could be obtained. Two possibilities of binding monochloroethylamine (MCA) to a polymer were investigated: (1) Copolymerization of N -vinyl pyrrolidone (VP) with monochloroethylamide of methacrylic acid (MCAMA). The constants of copolymerization of this pair: r = 3.88 & 0.31 and r 2 = 0.089 k 0.017 were determined, as well as the mean length of the elementary link of the copolymer chain. It was found that at a ratio of VP:MCAMA = 1:3 a copolymer of statistically probable structure could be obtained in which the distance between two monochloroethylamine groups is the most appropriate: about 6 atoms. (2) A polymer-analogous reaction of the copolymer of VP with maleic anhydride (MA) permitting the binding of MCA to the polymer chain by severing the MA ring. It was established that the reaction proceeded best in a medium of dimethylformamide at S O T for 1 hr. Water-soluble products characterized by their C1 content and ir spectra were obtained. These products showed a physiological activity.
The aim of our study was to investigate the possibility of forming the microbiota of the cecum of rabbits with increased content of cellulolytic bacteria Butirivibrio fibrisolvens, due to the transfer of the contents of the rumen of ruminants. To solve this problem, experimental (n=15) and control (n=15) groups of rabbits aged 12 days were formed. In the experimental group, for 14 days the rabbits were tube-feed with a solution of cattle rumen content filtered from coarse undigested feed particles in 1-2 ml diluted 1:1 in physiological solution. To determine the level of cellulosolytic bacteria Butirivibrio fibrisolvens, feces were collected in sterile eppendorfs on day 14 of the study. The presence of cellulosolytic bacteria in cattle rumen content as well as in rabbit feces was determined by real-time polymerase chain reaction. Real-time PCR results showed that the highest fluorescence values were in the rumen contents and at cycle 40 the fluorescence intensity was 1742.60, in the experimental group it was 1059.98 and in the control group without tube-feeding it was 664,50. Thus, it was shown that by feeding cattle rumen content in the early postnatal period in rabbits can form the gut microbiota with an increased content of cellulolytic bacteria Butirivibrio fibrisolvens.
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