Formation of microbiota in the chyme of the cecum of rabbits with increased content of cellulolytic bacteria

Abstract

The aim of our study was to investigate the possibility of forming the microbiota of the cecum of rabbits with increased content of cellulolytic bacteria Butirivibrio fibrisolvens, due to the transfer of the contents of the rumen of ruminants. To solve this problem, experimental (n=15) and control (n=15) groups of rabbits aged 12 days were formed. In the experimental group, for 14 days the rabbits were tube-feed with a solution of cattle rumen content filtered from coarse undigested feed particles in 1-2 ml diluted 1:1 in physiological solution. To determine the level of cellulosolytic bacteria Butirivibrio fibrisolvens, feces were collected in sterile eppendorfs on day 14 of the study. The presence of cellulosolytic bacteria in cattle rumen content as well as in rabbit feces was determined by real-time polymerase chain reaction. Real-time PCR results showed that the highest fluorescence values were in the rumen contents and at cycle 40 the fluorescence intensity was 1742.60, in the experimental group it was 1059.98 and in the control group without tube-feeding it was 664,50. Thus, it was shown that by feeding cattle rumen content in the early postnatal period in rabbits can form the gut microbiota with an increased content of cellulolytic bacteria Butirivibrio fibrisolvens.