Abstract. Hartati S, Nandariyah, Yunus A, Djoar DW. 2017. Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley). Biodiversitas 18: 555-559. Black orchid (Coelogyne pandurata Lindley) is one of the rare species of Coelogyne Lindley, protected by the government of Indonesia. The cytological character of orchid is very important to study to support the success of breeding. The study aimed to assess the chromosomal number, karyotype pattern and ploidy level the hybrid of Black Orchid (Coelogyne pandurata). This study shows that the chromosomal number of the hybrid of Coelogyne pandurata (2n = 36) >< Coelogyne rumphii (2n = 72) is 2n = 54. Ploidy analysis by flow cytometry shows that hybrid shows triploid (2n = 3x = 54) different from the parent Coelogyne pandurata which is diploid (2n = 2x = 36) and the parent Coelogyne rumphii which is tetraploid (2n = 4x = 72). However, both parents and their hybrid performed the same karyotype pattern, which is metacentric. The chromosome size showed a variation in length from the longest Coelogyne pandurata 2.98 ± 0.15 µm to the shortest Coelogyne rumphii 2.24 ± 0.15 µm. The hybrids have a range of 2.50 ± 0,10 µm to 2.85 ± 0.10 µm. Karyotype patterns of black orchid (C. pandurata), C. rumphii and the hybrid is metacentric.
Brown rice is a type of rice can be grown on land that is less water (upland). Brown rice has anthocyanin as an antioxidant. The purpose of this study was to identify brown rice agronomics characters that can be used as selection criteria to obtain brown rice which high anthocyanin. The experiment was conducted at the greenhouse of Agriculture Faculty, UNS using completed randomized block design consisting of 5 local varieties namely Mandel Wonogiri, Segreng Gunung Kidul, Segreng Boyolali, Segreng Wonogiri 1, and Segreng Wonogiri 2. Analysis of the data using the model Singh and Chaudhary (1979) are displayed in the form of a matrix correlation. Anthocyanin assays using the differential pH test method. The results showed that there was no difference between the anthocyanin content of varieties, but there is a tendency Segreng Boyolali varieties containing anthocyanins and Mandel Wonogiri higher. In general, varieties tested showed different potential yield, except Mandel Wonogiri varieties have low yield. The character of the total number of tillers, panicle length, and number of panicles per hill showed a strong correlation coefficient with the results. Plant height, number of panicles per hill, and the weight of 1000 seeds showed a high correlation coefficient with anthocyanin content.
A better understanding of variability in plant populations is crucial for crop improvement which plant breeders can use through selection. This study aimed to determine selection criteria for the sixth generation of black rice through genetic variability, heritability value and genetic advance. This research was conducted from March to July 2018 at Pakahan Village, Jogonalan Sub-district, Klaten Regency, Central Java, using a Randomized Complete Block Design (RCBD). The variables observed were plant height, total number of tillers and productive tillers, panicle length, number of seeds per panicle, panicle intensity index, weight per panicle, 1,000 seed weight, seed weight per plant, flowering age, harvest age, plot yield, yield per hectare, rice color and total anthocyanin content. Variance analysis showed that there was a very significant difference (p <0.01) among the genotypes tested for all observed characters indicating variability. In this study, the phenotypic coefficient of variance (PCV) values was relatively higher than the genotypic coefficient of variance (GCV) for all traits. However, GCV values were close to PCV values in some characters such as plant height, flowering age, harvest age, rice color and anthocyanin content which showed a high contribution of genotypic effects to the phenotypic expression of these characters. The high heritability and high average value of genetic advance were indicated in the parameters of plant height, flowering age, harvest age, rice color and anthocyanin content. The characters of plant height, flowering age, harvest age, rice color and anthocyanin content can be used as sixth-generation black rice selection criteria.
<p>Study Morphology and Correlation Analysis Inter Componen<br />Yield Caracter of Citronella (Cymbopogon citratus). Lemongrass isfamiliar in Indonesia as a cooking ingredient known as Lemongrass (Cymbopogon citratus). Citronella considered as a rare plant that most peopledo not recognize yet. There are two species of Citronella are known Citronella Lenabatu (Cymbopogon nardus) and Citronella Mahapengiri (Cymbopogon winterianus) which is an essential oil taken as a raw material tomake medicine and perfume. This research was conducted in BKPH Southern Lawu, RPH Watu kempul and consortium village of Sendang, district Jatipuro, Wonogiri on December 2010. The purpose of this research are to find morphology characteristic, yield of essential oil and the variety level of Citronella, that all used as the selection foundation to get a bit of blood. This research uses 30 samples/accessions based on Random Proportions. Observation is conducted on the characteristic of morphology and yield of essential oil, variety level is implemented based on the Cluster analysis with average linkage method. The results show that 24,90% variety of Citronella in the area is not too varied. This variation can be seen from the morphological characteristic, and the differences of yield of essential oil produced.Accessions 26 and accessions 24 have highest oil yield with 1.421% and1.25%. The two samples have similar in the morphology, which they have similar red to purple leaf color, similar in the minimum number of plants inone clump (50-100) and the tall of plant that not too high (150 cm-200 cm).Components of plant cintronella fragrant hat could be use as selection criteria including of low plant hight, shorter of leaf size, number of plants per hill slightly and Shorter of stem length.</p>
<p>The purpose of this study to determine the morphological characteristics and variability of planting sugar apple Gedangsari, Gunungkidul, Yogyakarta. Identify the morphological characteristics conducted on 36 plant samples (36 accessions) based method on stratified sampling and purposive sampling. The data were analyzed descriptively and clustering analysis. The results of the research showed that sugar-apple had pyramida canopy shape, plant height of 264±525 cm, oblongus leaves type, integer leaves margins, acuminatus leaves tip form, base acutus leaves, petiole cylindrical shape, dark green petiole, dark green color of the upper surface leaves, faded green color of the lower surface, leaves length 16.06±8.23 cm, leaves width 3,73±6,9 cm, leaves area 24,28±85,71 cm2, round shaped of rod, patens branching pattern, stem circumference 17,5±38 cm, axillary flos of flower layout, having 3 petals, whitish green of flower color, is cordate and round shape of fruit, fruit weight of 83,03±180,3 g, fruit circumference 22,4±19,36 cm, flesh thick 1,73±3,66 mm, whitish green and green of fruit color, the total amount of fruit scales 70±104,66, and the value of PTT between 18-29 obrix, blunt shape of fruit scales, ellipsoid shape of seeds, number seeds 7±70,66 and total seed weight 2,3±19,86g. There were variability include leaves length, leaves width, leaves area, stem girth, fruit weight, fruit girth, fruit flesh thickness, number of fruit scales, PTT values and fruit shape and the fruit skin’s color. The result of clustering analysis showed that similarity coefficient between the accessions was 66%.</p>
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