Short Communication: Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley)

Hartati, Sri; Nandariyah, Nandariyah; Yunus, Ahmad; Djoar, Djati Waluyo

doi:10.13057/biodiv/d180216

Cited by 21 publications

(23 citation statements)

References 9 publications

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“…Dendrogram shows that ten ISSR molecular markers are able to classify tree similarity clusters with a similarity coefficient of 0.51. The first cluster consisted of C. pandurata, C. rumphii, C. mayeriana, C. asperata, the second cluster was C. celebensis and the third was C. massangeana (Hartati et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Orchidaceae is one of the largest family of plants, consisting of more than 25,000 species. Orchids are recognized as economically important commodities in the international flower industry, either as cut flowers or potted plants (Chase 2005;Hartati et al, 2017;Kuehnle, 2007). In adition to being used as an ornamental plant, several types of orchids have also been used as food or medicine (Luo et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Morphological characterization of Coelogyne spp for germplasm conservation of orchids

et al. 2019

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Orchid is one of the ornamental plant that has a high aesthetic value. Efforts to increase the quality of orchids can be done by genetic improvement through crossing. The first step to success in orchid crossing requires information on morphological characters of the prospective parents. This study aims to determine the similarity of six species of natural Coelogyne spp based on qualitative morphological characters. Morphological characterization of 6 orchids was carried out based on 30 characters including stem, leaf, flower, pseudobulb, and rooting type. Cluster analysis was carried out with the NTSYSpc program version 2.02i with the UPGMA SimQual function method. The results showed that there were morphological diversities among the six Coelogyne spp on the character of the stem, pseudobulb, leaves, flowers and type of roots. Based on the dendrogram on 73% similarity, three major groups were obtained. The first group consisted of Coelogyne pandurata and Coelogyne rumphii, the second group was C. massangeana, C. mayeriana and C asperata, the third group was C. celebensis. Based on the morphological similarity of 87% there are two pairs of parents that have most successful chance to be crossed, C. pandurata with C. rumphii and C. mayeriana with C. asperata.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Morphological characterization of Coelogyne spp for germplasm conservation of orchids

et al. 2019

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“…Based on ISSR markers, C. pandurata and C. rumphii have the closest genetic relationship that is 70%. Additionally, cytological studies have shown that the karyotype of C. pandurata is 2n=56, C. rumphii is 2n=72 and their hybrid is 2n=54 (Hartati et al 2017). A second study showed that based on the morphological identification of these two species, they have a morphological similarity of 87% (Hartati et al 2019a).…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers

Hartati

Muliawati

2020

Biodiversitas

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Abstract. Hartati S, Muliawati ES. 2020. Short Communication: Genetic variation of Coelogyne pandurata, C. rumphii and their hybrids based on RAPD markers. Biodiversitas 21: 4709-4713. One effort to increase the genetic diversity of orchids is by crossing. This research aims to assess the genetic variation of a hybrid orchid obtained by crossing Coelogyne pandurata and C. rumphii and their hybrids based on RAPD markers. In this research, both parents were analyzed in three replications, while the hybrid was done in 10 replications. The study was conducted by analyzing DNA bands using RAPD markers with six primers, i.e. OPA 02, OPA 07, OPA 13, OPB 12, OPB 17, and OPD 08. Identification of the parents and their F1 hybrids showed 95.83% polymorphic bands with 43 bands measuring 200-2100 bp. The parents of C. rumphii, C. pandurata, and their hybrids showed similarity range of 0.16-1.00. The crossing of C. rumphii and C. pandurata resulted in a similarity of 0.5, shown in two large clusters. The first cluster consisted only of C. pandurata males and the second cluster consisted of C. rumphii females, together with all hybrid individuals. This study succeeded in creating new hybrids of orchids that have different characters from their parents, having a genetic variation of 23%.

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“…The 2n range from 28 in Canthigaster coronata (Vaillant & Sauvage, 1875) (Arai 2011) to 46 chromosomes in the genus Sphoeroides anonymous, 1798 (Brum et al 1995, Brum 2000, Sá-Gabriel and Molina 2005, Alves et al 2008). Shape, size, and number of chromosomes of each species are always fixed, so knowledge about the karyotypes can be used for studies in taxonomy, diversity, kinship, and evolution, even though in certain circumstances also variations have been reported (Hartati et al 2017). To identify intra-and inter-specific variants karyotyping combined with molecular cytogenetic analyses are the methods best suited here.…”

Section: Introductionmentioning

confidence: 99%

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

et al. 2020

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Abstract. Pissaparn M, Phimphan S, Chaiyasan P, Tanoamtong A, Liehr T, Suwannapoom C, Reungsing M, Supiwong W. 2020. First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866). Biodiversitas 21: 4309-4316. Here first analysis of chromosomes and nucleolar organizer region (NOR) pattern in pufferfish Pao cochinchinensis (Steindachner, 1866) was undertaken. Chromosomal preparations were obtained from kidney of P. cochinchinensis from Chi River basin in Thailand. Chromosomal characteristics were analyzed by Giemsa staining, Ag-NOR banding as well as fluorescence in situ hybridization (FISH) using microsatellites d(CA)15 and d(CGG)10 probes. P. cochinchinensis had 2n = 40 with the fundamental number (NF) 74, both in male and female. The karyotype exhibited 12 metacentric (m), 10 submetacentric (sm), 12 acrocentric (a) and 6 telocentric (t) chromosomes. No differentiated heteromorphic sex chromosomes were observed. NORs were located on short arms adjacent to telomere of the metacentric chromosome pair 4, which coincide with signals of d(CGG)10 probe. FISH with d(CGG)10 sequences were also displayed at the telomeres of most other chromosomes, whereas d(CA)15 repeats highly accumulated throughout almost all entire chromosomes except for centromeric regions. The results of conventional Giemsa staining presented the differentiation even the same genus. The localization of NORs on one pair of chromosomes only is a common characteristic found in many fish groups as well as other vertebrates. Mapping of two distinct microsatellites demonstrated the remarkable chromosomal diversification that characterizes evolution in the genus Pao. Both, conventional and molecular cytogenetics are excellent tools to study, and better understand chromosomal evolution, as well as to uncover biodiversity among fishes.

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Short Communication: Cytological studies on black orchid hybrid (Coelogyne pandurata Lindley)

Cited by 21 publications

References 9 publications

Morphological characterization of Coelogyne spp for germplasm conservation of orchids

Morphological characterization of Coelogyne spp for germplasm conservation of orchids

Short Communication: Genetic variation of Coelogyne pandurate, C. rumphii and their hybrids based on RAPD markers

First chromosome analysis of Thai pufferfish Pao cochinchinensis (Steindachner, 1866)

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