Some regions in Indonesia, especially East Java, have abundant local varieties of rice. However, these germplasms have not been optimally developed in plant breeding. Characterization ranked as the first step in plant breeding to identify morphological and agronomic characteristics of the germplasms. This study aims to characterize several local rice varieties in East Java, including ‘Ketan Genjah’ (V1), ‘PIM’ (V2), ‘Pendok’ (V3), ‘Padi Hitam’ (V4), and ‘Padi Merah’ (V5) to point out each strength in several parameters to be used as a potential gene source for plant breeding. The results of this study indicated that the varieties studied have several distinct morphological and agronomic characters with each other. In terms of plant height, leaf length, pithy grain percentage, grain loss percentage, and the weight of 1000 grain, ‘Pendok’ showed superiorities compared to other varieties. In addition, ‘Pendok’ also had the highest yield per clump together with ‘Ketan Genjah’ at 151.32 g and 150.82 g, respectively. ‘PIM’ had the longest panicle length, whereas in terms of number of tillers, ‘Ketan Genjah’, ‘Padi Hitam’, and ‘Padi Merah’ were the highest among the varieties studied.
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