Rheedia longifolia Planch et Triana belongs to the Clusiaceae family. This plant is widely distributed in Brazil, but its chemical and pharmacological properties have not yet been studied. We report here that leaves aqueous extract of R. longifolia (LAE) shows analgesic and anti-inflammatory effects. Oral or intraperitoneal administration of this extract dose-dependently inhibited the abdominal constrictions induced by acetic acid in mice. The analgesic effect and the duration of action were similar to those observed with sodium diclofenac, a classical non-steroidal analgesic. In addition to the effect seen in the abdominal constriction model, LAE was also able to inhibit the hyperalgesia induced by lipopolysaccharide from gram-negative bacteria (LPS) in rats. We also found that R. longifolia LAE inhibited an inflammatory reaction induced by LPS in the pleural cavity of mice. Acute toxicity was evaluated in mice treated with the extract for seven days with 50 mg/kg/day. Neither death, nor alterations in weight, blood leukocyte counts or hematocrit were noted. Our results suggest that aqueous extract from R. longifolia leaves has analgesic and anti-inflammatory activity with minimal toxicity and are therefore endowed with a potential for pharmacological control of pain and inflammation.
Different species of the family Clusiaceae, including Rheedia longifolia, are used in folk medicine to treat inflammatory diseases. This family is largely distributed in tropical and subtropical areas of Brazil, but their chemical and pharmacological properties have been the subject of a few studies. In previous studies, we found that the aqueous extract from R. longifolia leaves presented important anti-inflammatory and analgesic activity. We investigated the chemical profile of R. longifolia and characterized the pharmacological effect of different chemically identified fractions in pharmacological models of neurogenic and inflammatory nociception. The pharmacological tests showed that oral treatment with aqueous crude extract and fractions of methanol extract of R. longifolia leaf induced a significant antinociceptive effect using von Frey filaments. In addition, the most polar fractions presented antinociceptive activity in a neurogenic model of nociception (capsaicin model). The chromatographic analysis indicated the presence of bisflavonoids in the fractions obtained from the methanol extract. These results suggest that bisflavonoids found in methanol-extracted fractions are involved in the inhibition of inflammatory and neurogenic nociception. It is important that the R. longifolia aqueous extract treatment inhibited ulcer formation induced by indomethacin, suggesting an anti-ulcerogenic activity closely associated with its analgesic effect.
A tuberculose assola a humanidade desde os primórdios como grande problema de saúde global, impactando cerca de 10 milhões de pessoas a cada ano e sendo uma das dez principais causas de morte em todo o mundo. Tratamentos eficazes iniciaram em 1940. No Brasil, é um importante problema de saúde pública influenciado pelos determinantes sociais. A Organização Mundial da Saúde lançou o programa End TB Strategy visando acabar com a epidemia da TB no mundo até 2035. O tratamento eficaz da TB reúne uma combinação de antimicrobianos com atividade contra o M. tuberculosis, como a associação de isoniazida, rifampicina, pirazinamida e etambutol. No entanto, devido ao esquema posológico extenso, há uma baixa adesão do paciente ao tratamento, aumentando os riscos de morbidade, mortalidade e resistência microbiana aos medicamentos. Assim, associações em dose fixa combinada (DFC) simplificam o esquema terapêutico e favorecem a adesão dos pacientes ao tratamento. Porém, um dos desafios para o desenvolvimento de medicamentos em DFC para o tratamento da tuberculose é manter a qualidade da DFC até o fim de validade pelos estudos de estabilidade comprovados conforme a RDC 318/2019 da ANVISA. Este trabalho visa refletir sobre a problemática dos produtos de degradação na DFC e contribuir para observância de melhores métodos indicativos de estabilidade.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.