Filters are fundamental components widely used in Electric Information and Control Engineering. Filters based on rectangular waveguide have good performance with low loss, high operation frequencies and high power. But the design and optimizing is a rather complex job. In this paper, a neural network is introduced to optimize two parameters in a waveguide band-pass filter. The result shows that after training, the neural network can predict the S parameter well and make the process of optimizing easier.
Keywords-rectangular waveguide; back-propagation neural network; band-pass filtersI.
Quorum sensing, as inner- or inter-species microbial communication process orchestrated by diffusible autoinducers, typically results in collective pathogenic behaviours, being recognized as a promising druggable target for anti-virulence. Here, we reconstituted las and rhl quorum sensing pathways of Pseudomonas aeruginosa, mediated by acyl-homoserine lactones (AHLs) and LuxI/LuxR-family proteins, with fluorescence output in Escherichia coli cell-free expression system, offering a platform to rapidly evaluate quorum sensing inhibitors (QSIs) in vitro. Previously reported small-molecule quorum sensing inhibitors for interfering with P. aeruginosa quorum sensing systems were tested and showed mild to high on-target inhibition as well as off-target toxicity. Of note, quercetin displayed potent on-target inhibition to quorum sensing pathways as well as acceptable off-target toxicity to cell-free expression machinery. Upon our work, cell-free platform is anticipated to further facilitate automated and high-throughput drug screening, bridge in silico and in vivo drug-screening methods, and accelerate the upgrading of antimicrobial arsenal.GRAPHICAL ABSTRACT
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